Abdolmohammad-Zadeh Hossein, Ahmadian Farzaneh
Analytical Spectroscopy Research Lab., Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, 35 Km Tabriz-Marageh Road, P.O. Box 53714-161, Tabriz, 5375171379, Iran.
Analytical Spectroscopy Research Lab., Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, 35 Km Tabriz-Marageh Road, P.O. Box 53714-161, Tabriz, 5375171379, Iran.
Anal Chim Acta. 2023 May 1;1253:341055. doi: 10.1016/j.aca.2023.341055. Epub 2023 Mar 7.
Organophosphorus pesticides are widely used in agriculture owing to their high effectiveness as insecticides. Among these, diazinon is a common environmental contaminant that acts as an acetylcholinesterase (AChE) enzyme inhibitor. As the current methods are too expensive and time-consuming for routine analysis of diazinon, its trace monitoring by rapid and sensitive methods is critical to protect the environment and human health.
A biosensor was introduced for the indirect detection of diazinon using a molybdenum disulfide/zirconium metal-organic framework (MoS@MIP-202(Zr)) nanocomposite. The probe is based on the peroxidase mimic of the prepared nanocomposite on NaHCO-HO chemiluminescence system as well as the inhibitory effect of diazinon on the enzymatic activity of AChE. The chemiluminescence signal is gradually decreased with an increase in diazinon concentration, and there is a linear relationship between the analytical signal and diazinon concentration. Under the optimum conditions, the calibration plot is linear in the concentration range of 0.5-300.0 nmol L. The limit of detection and quantification limit of the method are 0.12 and 0.40 nmol L, respectively. The inter-day and intra-day relative standard deviations (% RSD n = 5, diazinon concentration; 100 nmol L) are 3.66 and 1.35%, respectively. The method was used for diazinon detection in real water samples, and the high relative recovery values for the spiked samples along with satisfactory results of a certified reference material analysis confirmed that the method is accurate and free from the matrix effect.
A nano-probe based on the peroxidase-like property of MoS@MIP-202(Zr) nanocomposite was developed for the first time for indirect detection of residue levels of diazinon in water samples. The high stability of the nanocomposite makes it a good alternative for natural peroxidase enzymes such as horseradish peroxidase with low stability.
有机磷农药因其作为杀虫剂的高效性而被广泛应用于农业。其中，二嗪农是一种常见的环境污染物，它作为乙酰胆碱酯酶（AChE）抑制剂发挥作用。由于目前的方法对于二嗪农的常规分析过于昂贵且耗时，通过快速灵敏的方法对其进行痕量监测对于保护环境和人类健康至关重要。
引入了一种生物传感器，用于使用二硫化钼/锆金属有机框架（MoS@MIP-202(Zr)）纳米复合材料间接检测二嗪农。该探针基于制备的纳米复合材料在NaHCO-HO化学发光体系上的过氧化物酶模拟特性以及二嗪农对AChE酶活性的抑制作用。随着二嗪农浓度的增加，化学发光信号逐渐降低，且分析信号与二嗪农浓度之间存在线性关系。在最佳条件下，校准曲线在0.5-300.0 nmol L的浓度范围内呈线性。该方法的检测限和定量限分别为0.12和0.40 nmol L。日间和日内相对标准偏差（%RSD，n = 5，二嗪农浓度；100 nmol L）分别为3.66%和1.35%。该方法用于实际水样中二嗪农的检测，加标样品的高相对回收率值以及有证标准物质分析的满意结果证实了该方法准确且无基质效应。
首次开发了基于MoS@MIP-202(Zr)纳米复合材料类过氧化物酶特性的纳米探针，用于间接检测水样中二嗪农的残留水平。纳米复合材料的高稳定性使其成为稳定性低的天然过氧化物酶（如辣根过氧化物酶）的良好替代品。
