一种用于检测活细胞中次氯酸的苯并噻唑基荧光探针及成像。
A benzothiazole-based fluorescent probe for hypochlorous acid detection and imaging in living cells.
机构信息
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, Hunan 476000, PR China.
Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, Hunan 476000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jun 15;199:189-193. doi: 10.1016/j.saa.2018.03.055. Epub 2018 Mar 24.
A benzothiazole-based turn-on fluorescent probe with a large Stokes shift (190nm) has been developed for hypochlorous acid detection. The probe displays prompt fluorescence response for HClO with excellent selectivity over other reactive oxygen species as well as a low detection limit of 0.08μM. The sensing mechanism involves the HClO-induced specific oxidation of oxime moiety of the probe to nitrile oxide, which was confirmed by HPLC-MS technique. Furthermore, imaging studies demonstrated that the probe is cell permeable and can be applied to detect HClO in living cells.
一种基于苯并噻唑的荧光探针，具有较大的斯托克斯位移（190nm），已被开发用于检测次氯酸。该探针对 HClO 具有快速的荧光响应，对其他活性氧具有优异的选择性，检测限低至 0.08μM。传感机制涉及探针肟部分被 HClO 特异性氧化为亚硝酮，这一点通过 HPLC-MS 技术得到了证实。此外，成像研究表明，该探针具有细胞通透性，可用于检测活细胞中的 HClO。
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