Çetin Esin Sakallı, Sozen Hamdi, Celik Ozgur Ilhan, Cigerci Ibrahim Hakkı, Yılmaz Nigar
Department of Medical Biology, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
Department of Infectious Disease, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
Pak J Pharm Sci. 2023 Jan;36(1):9-16.
In the current study, the protective effect of a mistletoe extract (Helixor®, HLX) on Itraconazole (ITZ)-induced hepatocellular injury and acute oxidative stress in rats was aimed to be investigated by histological, biochemical and comet assay methods. Four groups a control group, an HLX group (5mg/kg/14days/intraperitoneally (ip)), an ITZ group (100mg/kg/14days/oral) and an HLX plus ITZ group (5mg/kg/14days/ip+100mg/kg/14days/oral) were all created from 32 female Wistar albino rats. At the end of the experiment, AST and ALT liver enzymes, total oxidant status (TOS) levels and total antioxidant status (TAS) levels, histopathological analysis and comet assay were carried out. Highest genotoxicity, higher levels of plasma AST and ALT, higher TOS, more degeneration of liver histopathology including hepatocyte degeneration, hepatocyte apoptosis and necrosis, portal/periportal inflammation, bile ductus hyperplasia and multinuclear giant cell formation were observed in ITZ group (p<0.05). As opposed to that, administration of HLX plus ITZ improved histopathological changes and DNA damage and showed a dramatic decrease in AST, ALT and TOS levels (p<0.05) and an increase in TAS level (p<0.001) when compared to ITZ group. This study showed that the antioxidant properties of HLX administration significantly decreased acute oxidative stress and hepatocellular damage in rats given ITZ.
在本研究中，旨在通过组织学、生化和彗星试验方法，研究一种槲寄生提取物（Helixor®，HLX）对大鼠伊曲康唑（ITZ）诱导的肝细胞损伤和急性氧化应激的保护作用。从32只雌性Wistar白化大鼠中创建了四组：对照组、HLX组（5mg/kg/14天/腹腔注射(ip)）、ITZ组（100mg/kg/14天/口服）和HLX加ITZ组（5mg/kg/14天/腹腔注射+100mg/kg/14天/口服）。实验结束时，进行了AST和ALT肝酶、总氧化剂状态（TOS）水平和总抗氧化剂状态（TAS）水平、组织病理学分析和彗星试验。在ITZ组中观察到最高的遗传毒性、血浆AST和ALT水平升高、TOS升高、肝组织病理学更多的退变，包括肝细胞变性、肝细胞凋亡和坏死、门静脉/门静脉周围炎症、胆管增生和多核巨细胞形成（p<0.05）。与此相反，与ITZ组相比，HLX加ITZ给药改善了组织病理学变化和DNA损伤，并使AST、ALT和TOS水平显著降低（p<0.05），TAS水平升高（p<0.001）。本研究表明，HLX给药的抗氧化特性显著降低了给予ITZ的大鼠的急性氧化应激和肝细胞损伤。
