Sedik Ahmed A, Hassan Azza, Salama Abeer
Pharmacology Department, Medical Research and Clinical Studies Institute, National Research Centre, 12622, Egypt.
Pathology Department, Faculty of Veterinary Medicine, Cairo University.
Iran J Basic Med Sci. 2023;26(8):941-952. doi: 10.22038/IJBMS.2023.68855.15108.
Our study was conducted to evaluate the synergistic effect of arginine (ARG) and against potassium dichromate (K2Cr2O7) induced-acute hepatic and kidney injury.
Fifty male Wistar rats were divided into five groups. The control group received distilled water. The potassium dichromate group (PDC) received a single dose of PDC (20 mg/kg; SC). The arginine group (ARG) and group received either daily doses of ARG (100 mg/kg, PO) or (10 CFU/ml, PO) for 14 days. The combination group (ARG+) received daily doses of ARG (100 mg/kg) with (10 CFU/ml), orally for 14 days, before induction of acute liver and kidney injury. Forty eight hours after the last dose of PDC, serum biochemical indices, oxidative stress biomarkers, pro-inflammatory cytokines, histopathological and immunohistochemical analysis were evaluated.
Combining ARG with restored the levels of serum hepatic & kidney enzymes, hepatic & renal oxidative stress biomarkers, and TLR 4/ NF-κB signaling pathway. Furthermore, they succeeded in decreasing the expression of iNOS and ameliorate the hepatic and renal markers of apoptosis: Caspase-3, Bax, and Bcl2.
This study depicts that combining ARG with exerted a new bacteriotherapy against hepatic and renal injury caused by PDC.
本研究旨在评估精氨酸（ARG）和[具体物质未给出]对重铬酸钾（K2Cr2O7）诱导的急性肝损伤和肾损伤的协同作用。
将50只雄性Wistar大鼠分为五组。对照组给予蒸馏水。重铬酸钾组（PDC）接受单次剂量的PDC（20mg/kg；皮下注射）。精氨酸组（ARG）和[具体物质未给出]组分别接受每日剂量的ARG（100mg/kg，口服）或[具体物质未给出]（10CFU/ml，口服），持续14天。联合组（ARG+）在诱导急性肝损伤和肾损伤前，接受每日剂量的ARG（100mg/kg）与[具体物质未给出]（10CFU/ml）口服，持续14天。在最后一剂PDC给药48小时后，评估血清生化指标、氧化应激生物标志物、促炎细胞因子、组织病理学和免疫组织化学分析。
将ARG与[具体物质未给出]联合使用可恢复血清肝酶和肾酶水平、肝和肾氧化应激生物标志物以及TLR 4/NF-κB信号通路。此外，它们成功降低了诱导型一氧化氮合酶的表达，并改善了凋亡的肝和肾标志物：半胱天冬酶-3、Bax和Bcl2。
本研究表明，将ARG与[具体物质未给出]联合使用可对PDC引起的肝损伤和肾损伤产生新的细菌疗法。
