Department of Chemistry, University of Balochistan, Sariab Road, Quetta, Pakistan.
Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Marti i Franques 1-11, Barcelona, Spain.
Luminescence. 2023 May;38(5):647-661. doi: 10.1002/bio.4493. Epub 2023 Apr 10.
A flow injection (FI) methodology using the acidic potassium permanganate (KMnO )-rhodamine-B (Rh-B) reaction with chemiluminescence (CL) detection was established to determine acetochlor and cartap-HCl pesticides in freshwater samples. Experimental parameters were optimized, and Chelex-100 cationic exchanger mini column and solid-phase extraction (SPE) were used as phase separation techniques. Linear calibration curves were observed for the standard solutions of acetochlor and cartap-HCl over the ranges 0.005-2.0 mg L [y = 1155.8x + 57.551, R = 0.9999 (n = 8)] and 0.005-1.0 mg L [y = 979.76x + 14.491, R = 0.9998 (n = 8)] with LODs and LOQs of 7.5 × 10 and 8.0 × 10 mg L (3σ blank) and 2.5 × 10 and 2.7 × 10 mg L (10σ blank), respectively, with an injection throughput of 140 h . These methods were used to estimate acetochlor and cartap-HCl with or without the SPE procedure, respectively, in spiked freshwater samples. Results obtained were not significantly different at a 95% confidence level to those of other reported methods. Recoveries for acetochlor and cartap-HCl were obtained over the ranges 93-112% (RSD = 1.9-3.6%) and 98-109% (RSD = 1.7-3.8%), respectively. The most probable CL reaction mechanism was explored.
建立了一种利用酸性高锰酸钾（KMnO ）-罗丹明 B（Rh-B）反应与化学发光（CL）检测的流动注射（FI）方法，用于测定淡水样品中的乙草胺和杀虫脒盐酸盐农药。优化了实验参数，并使用 Chelex-100 阳离子交换剂微型柱和固相萃取（SPE）作为相分离技术。乙草胺和杀虫脒盐酸盐标准溶液在 0.005-2.0 mg/L 范围内呈线性校准曲线 [y=1155.8x+57.551，R 2 =0.9999（n=8）] 和 0.005-1.0 mg/L [y=979.76x+14.491，R 2 =0.9998（n=8）]，检出限和定量限分别为 7.5×10 和 8.0×10 毫克/升（3σ空白）和 2.5×10 和 2.7×10 毫克/升（10σ空白），进样通量为 140 小时。这些方法分别用于估计未经 SPE 程序和经 SPE 程序处理的加标淡水样品中的乙草胺和杀虫脒盐酸盐。在 95%置信水平下，所得结果与其他报道的方法无显著差异。乙草胺和杀虫脒盐酸盐的回收率分别为 93-112%（RSD=1.9-3.6%）和 98-109%（RSD=1.7-3.8%）。探讨了最可能的 CL 反应机制。
