Yang Xiao-Feng, Wu Dong-Bing, Li Hua
Institute of Analytical Science, Northwest University, Xi'an 710069, People's Republic of China.
Luminescence. 2004 Nov-Dec;19(6):322-7. doi: 10.1002/bio.781.
A novel flow injection chemiluminescence (CL) method for the determination of dihydralazine sulphate (DHZS) is described. The method is based on the CL produced during the oxidation of DHZS by acidic permanganate solution in the presence of rhodamine B. Rhodamine B is suggested as a fluorescing compound for the energy-transferred excitation. The CL emission allows quantitation of DHZS concentration in the range 5-800 ng/mL, with a detection limit of 1.9 ng/mL (3sigma). The experimental conditions for the CL reaction are optimized and the possible reaction mechanism is discussed. The method has been applied to the determination of DHZS in pharmaceutical preparations and compares well with the high performance liquid chromatography (HPLC) method.
描述了一种用于测定硫酸肼屈嗪（DHZS）的新型流动注射化学发光（CL）方法。该方法基于在罗丹明B存在下，酸性高锰酸钾溶液氧化DHZS过程中产生的化学发光。罗丹明B被建议作为用于能量转移激发的荧光化合物。化学发光发射可对5 - 800 ng/mL范围内的DHZS浓度进行定量，检测限为1.9 ng/mL（3σ）。优化了化学发光反应的实验条件，并讨论了可能的反应机理。该方法已应用于药物制剂中DHZS的测定，与高效液相色谱（HPLC）法相比效果良好。
