Ramli Rabiatul Amirah, Mohamad Razali Umi Hartina, Izzreen Mohd Noor Nor Qhairul
Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
Heliyon. 2023 Mar 7;9(3):e14367. doi: 10.1016/j.heliyon.2023.e14367. eCollection 2023 Mar.
The present study was to determine optimum conditions for gelatin extraction from the skin of buffalo () using response surface methodology. A central composite design (CCD) was performed to evaluate the effects of NaOH concentration ( ), pre-treatment time ( ), extraction temperature ( ), and extraction time ( ) on the yield ( ), gel strength ( ), and hydroxyproline content ( ) of the extracted gelatin. The optimal combination of the independent variables for a good gelatin yield with high gel strength and hydroxyproline content was found at (0.77 M), (5.08 h), (62.93 °C) and (11.62 h). The experimental values for (16.91%), (236.5 g), and (41.4 g/100 g) were in good agreement with the predicted values of 17.87% yield, 237.80 g gel strength and 41.90 g/100 g of hydroxyproline content. Extraction temperature and extraction time were observed to be the most important factors that influenced the yield, gel strength, and hydroxyproline content, meanwhile pre-treatment time showed negative correlations with the yield and hydroxyproline content of the extracted gelatin. This study demonstrated that manipulation of specific parameters could improve extraction efficiency without compromising the quality of buffalo gelatin, thereby promoting it as an alternative source for gelatin production.
本研究旨在采用响应面法确定从水牛皮肤中提取明胶的最佳条件。进行了中心复合设计（CCD），以评估氢氧化钠浓度（ ）、预处理时间（ ）、提取温度（ ）和提取时间（ ）对提取明胶的产率（ ）、凝胶强度（ ）和羟脯氨酸含量（ ）的影响。发现对于具有高凝胶强度和羟脯氨酸含量的良好明胶产率，自变量的最佳组合为 （0.77 M）、 （5.08 h）、 （62.93 °C）和 （11.62 h）。 （16.91%）、 （236.5 g）和 （41.4 g/100 g）的实验值与预测值（产率17.87%、凝胶强度237.80 g和羟脯氨酸含量41.90 g/100 g）高度吻合。观察到提取温度和提取时间是影响产率、凝胶强度和羟脯氨酸含量的最重要因素，同时预处理时间与提取明胶的产率和羟脯氨酸含量呈负相关。本研究表明，操纵特定参数可以提高提取效率，而不影响水牛明胶的质量，从而将其推广为明胶生产的替代来源。
