Tadera Kayoko, Kitagawa Hiroki, Kitano Hiroyuki, Hara Toshinori, Kashiyama Seiya, Nomura Toshihito, Omori Keitaro, Shigemoto Norifumi, Yokozaki Michiya, Ohge Hiroki
Section of Clinical Laboratory, Division of Clinical Support, Hiroshima University Hospital, Hiroshima, Japan.
Division of Laboratory Medicine, Hiroshima University Hospital, Hiroshima, Japan.
Heliyon. 2023 Mar 11;9(3):e14543. doi: 10.1016/j.heliyon.2023.e14543. eCollection 2023 Mar.
, , and are commensal bacteria that are associated with colonization and infection of the urogenital tract. However, colonization of the respiratory tract by these microorganisms in adults has not been fully investigated.
Urine and respiratory tract samples (sputum, tracheal aspirates, and bronchoalveolar lavage) of patients aged 20-80 years were analyzed to detect the presence of , , and using a conventional PCR method. The samples were submitted to the microbiological clinical laboratory of Hiroshima University Hospital from December 1, 2021 to May 31, 2022.
In total, 334 urine and 238 respiratory tract samples were analyzed. The overall detection rates of , , and were 2.9%, 1.7%, and 2.3% in male urine; 7.0%, 13.8%, and 1.9% in female urine; 2.2%, 0%, and 2.2% in male respiratory tract; and 0%, 2.0%, and 0% in female respiratory tract, respectively. In urine samples, the detection rates of , , and were significantly higher (p < 0.001) for women (29/159; 18.2%) than for men (10/175; 5.7%); however, in respiratory tract samples, the detection rates were not significantly different (p = 0.70) between women (2/101; 2.0%) and men (5/137; 3.7%). Further, both the urine and respiratory samples of 83 patients were analyzed. Three male samples were positive for or , and and were matched in both the urine and respiratory tract samples: (n = 1), (n = 1), + (n = 1).
, , and were detected in the respiratory tract of not only the young patients, but also of patients aged 50-60 years. Further studies are required to understand the relationship of these microorganisms in urogenital and respiratory tract samples with extra-genital infections.
[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]是共生细菌，与泌尿生殖道的定植和感染有关。然而，这些微生物在成人呼吸道中的定植情况尚未得到充分研究。
对年龄在20 - 80岁患者的尿液和呼吸道样本（痰液、气管吸出物和支气管肺泡灌洗）进行分析，采用常规PCR方法检测[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]的存在情况。这些样本于2021年12月1日至2022年5月31日提交至广岛大学医院微生物临床实验室。
总共分析了334份尿液样本和238份呼吸道样本。[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]在男性尿液中的总体检出率分别为2.9%、1.7%和2.3%；在女性尿液中分别为7.0%、13.8%和1.9%；在男性呼吸道中分别为2.2%、0%和2.2%；在女性呼吸道中分别为0%、2.0%和0%。在尿液样本中，[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]在女性（共159例，29例阳性；18.2%）中的检出率显著高于男性（共175例，10例阳性；5.7%）（p < 0.001）；然而，在呼吸道样本中，女性（共101例，2例阳性；2.0%）和男性（共137例，5例阳性；3.7%）之间的检出率无显著差异（p = 0.70）。此外，对83例患者的尿液和呼吸道样本均进行了分析。有3份男性样本[具体细菌名称1]或[具体细菌名称2]呈阳性，且[具体细菌名称1]和[具体细菌名称2]在尿液和呼吸道样本中匹配：[具体细菌名称1]（n = 1）、[具体细菌名称2]（n = 1）、[具体细菌名称1] + [具体细菌名称2]（n = 1）。
不仅在年轻患者的呼吸道中检测到了[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]，在50 - 60岁患者的呼吸道中也检测到了。需要进一步研究以了解这些微生物在泌尿生殖道和呼吸道样本中与生殖道外感染的关系。
