Skiljevic Dusan, Mirkov Damjan, Vukicevic Jelica
Department of Dermatovenereology, Faculty of Medicine, University of Belgrade; Clinic of Dermatovenereology, Clinical Center of Serbia, Belgrade, Serbia.
Indian J Dermatol Venereol Leprol. 2016 Jan-Feb;82(1):37-41. doi: 10.4103/0378-6323.172903.
Mycoplasma hominis and Ureaplasma urealyticum are implicated in a wide array of infectious diseases in adults and children. Since some species have innate or acquired resistance to certain types of antibiotics, antibiotic susceptibility testing of mycoplasma isolated from the urogenital tract assumes increasing importance.
To evaluate the prevalence and antibiotic susceptibility of M. hominis and U. urealyticum in genital samples collected between 2007 and 2012.
Three hundred and seventy three patients presenting with symptoms of sexually transmitted diseases, infertility or risky sexual behaviour, who had not taken antibiotics in the previous 6 weeks and had ≥10 WBC per high power field on genital smears were studied. Urethral samples were taken in men and endocervical samples in women. The mycoplasma IST-2 kit was used for organism identification and for testing susceptibility to doxycycline, josamycin, ofloxacin, erythromycin, tetracycline, ciprofloxacin, azithromycin, clarithromycin and pristinamycin.
U. urealyticum was isolated from 42 patients and M. hominis from 11 patients. From 9.8% of isolates, both organisms were grown. All M. hominis isolates were resistant to tetracycline, clarithromycin and erythromycin while U. urealyticum was highly resistant to clarithromycin (94.6%), tetracycline (86.5%), ciprofloxacin (83.8%) and erythromycin (83.8%). M. hominis was sensitive to doxycycline (83.3%) and ofloxacin (66.7%) while most U. urealyticum strains were sensitive to doxycycline (94.6%).
Inability of the commercial kit used in the study to detect other potentially pathogenic urogenital mycoplasmas (Ureaplasma parvum, Mycoplasma genitalium).
There is significant resistance of U. urealyticum and M. hominis to tetracycline and macrolides. The most active tetracycline for genital mycoplasmas was found to be doxycycline, which continues to be the drug of first choice.
人型支原体和解脲脲原体与成人及儿童的多种传染病有关。由于某些菌株对某些类型的抗生素具有天然或获得性耐药性，因此对从泌尿生殖道分离出的支原体进行抗生素敏感性测试变得越来越重要。
评估2007年至2012年间收集的生殖器样本中人型支原体和解脲脲原体的患病率及抗生素敏感性。
对373例有性传播疾病症状、不孕或有危险性行为的患者进行研究，这些患者在过去6周内未服用过抗生素，且生殖器涂片每高倍视野白细胞≥10个。男性取尿道样本，女性取宫颈内膜样本。使用支原体IST - 2试剂盒进行菌种鉴定以及检测对多西环素、交沙霉素、氧氟沙星、红霉素、四环素、环丙沙星、阿奇霉素、克拉霉素和 pristinamycin 的敏感性。
从42例患者中分离出解脲脲原体，从11例患者中分离出人型支原体。9.8%的分离株中两种菌都生长。所有人型支原体分离株对四环素、克拉霉素和红霉素耐药，而解脲脲原体对克拉霉素（94.6%）、四环素（86.5%）、环丙沙星（83.8%）和红霉素（83.8%）高度耐药。人型支原体对多西环素（83.3%）和氧氟沙星（66.7%）敏感，而大多数解脲脲原体菌株对多西环素（94.6%）敏感。
研究中使用的商业试剂盒无法检测其他潜在致病性泌尿生殖道支原体（微小脲原体、生殖支原体）。
解脲脲原体和人型支原体对四环素和大环内酯类药物有显著耐药性。发现对生殖器支原体最有效的四环素类药物是多西环素，它仍然是首选药物。
