Sun Pei, Zhang Yuanyuan, Wang Chaoyue, Hu Dandan, Liu Jie, Chen Linlin, Shi Fangyun, Tang Xinming, Hao Zhenkai, Suo Jingxia, Yu Yonglan, Suo Xun, Liu Xianyong
National Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture & Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, China.
iScience. 2023 Mar 4;26(4):106334. doi: 10.1016/j.isci.2023.106334. eCollection 2023 Apr 21.
The control of coccidiosis, causing huge economic losses in the poultry industry, is facing the stagnation of the development of new drugs and the emergence of drug resistance. Thus, the priority for coccidiosis control is to decipher the effect mechanisms and resistance mechanisms of anticoccidial drugs. In this study, we mined and validated a molecular marker for halofuginone resistance in through forward and reverse genetic approaches. We screened whole-genome sequencing data and detected point mutations in the ETH2_1020900 gene (encoding prolyl-tRNA synthetase, PRS). Then, we introduced this mutated gene into and and validated that overexpression of this mutated gene confers resistance to halofuginone and . These results together show that mutations A1852G and A1854G on the ETH2_1020900 gene are pivotal to halofuginone resistance in , encouraging the exploration of mechanisms of drug resistance against other anticoccidial drugs in eimerian parasites.
球虫病的控制在家禽业中造成了巨大的经济损失，目前正面临着新药研发停滞和耐药性出现的问题。因此，控制球虫病的首要任务是破解抗球虫药物的作用机制和耐药机制。在本研究中，我们通过正向和反向遗传学方法挖掘并验证了一个针对常山酮耐药性的分子标记。我们筛选了全基因组测序数据，并检测到ETH2_1020900基因（编码脯氨酰-tRNA合成酶，PRS）中的点突变。然后，我们将这个突变基因导入[具体物种1]和[具体物种2]，并验证了该突变基因的过表达赋予了对常山酮[具体物种1]和[具体物种2]的抗性。这些结果共同表明，ETH2_1020900基因上的A1852G和A1854G突变对[具体物种]的常山酮耐药性至关重要，这鼓励了对艾美耳属寄生虫中其他抗球虫药物耐药机制的探索。
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