Chen Qi, Kuang Anchan, Wu Haihong, Liu Di, Zhang Xin, Mao Hongyu
College of Forestry, Shenyang Agricultural University, Shenyang, China.
Institute of Flowers, Liaoning Academy of Agricultural Sciences, Shenyang, China.
Front Plant Sci. 2023 Mar 10;14:1140596. doi: 10.3389/fpls.2023.1140596. eCollection 2023.
Chrysanthemum White Rust (CWR) caused by Henn. is a major disease in the production process of chrysanthemum, which is widely spread all over the world and can be called "cancer" of chrysanthemum. To clarify the disease resistance function of disease resistance genes can provide a theoretical basis for the utilization and genetic improvement of chrysanthemum resistant varieties. In this study, the resistant cultivar 'China Red' was used as the experimental material. We constructed the silencing vector pTRV2-CmWRKY15-1 and obtained the silenced line named TRV-CmWRKY15-1. The results of enzyme activity after inoculation with pathogenic fungi showed that the activities of antioxidant enzymes SOD, POD, CAT and defense-related enzymes PAL and CHI in leaves were stimulated under the stress of . . In the WT, the activities of SOD, POD and CAT at the peak value were 1.99 times, 2.84 times and 1.39 times higher than that in TRV-CmWRKY15-1, respectively. And the activities of PALand CHI at the peak were 1.63 times and 1.12 times of TRV-CmWRKY15-1. The content of MDA and soluble sugar also confirmed that chrysanthemum was more susceptible to pathogenic fungi when was silenced. The expression levels of , , and at different time points showed that the expressions of defense enzyme related genes were inhibited in TRV-WRKY15-1 under the infection of , which weakened the ability of chrysanthemum to resist white rust. In conclusion, may increased the resistance of chrysanthemum to white rust by increasing the activity of protective enzyme system, which laid a foundation for breeding new varieties with disease resistance.
由Henn.引起的菊花白锈病（CWR）是菊花生产过程中的一种主要病害，在世界各地广泛传播，堪称菊花的“癌症”。阐明抗病基因的抗病功能可为菊花抗病品种的利用和遗传改良提供理论依据。本研究以抗病品种‘中国红’为实验材料。构建了沉默载体pTRV2-CmWRKY15-1，并获得了名为TRV-CmWRKY15-1的沉默株系。接种病原菌后的酶活性结果表明，在……胁迫下，叶片中抗氧化酶SOD、POD、CAT以及防御相关酶PAL和CHI的活性受到刺激。在野生型中，SOD、POD和CAT活性峰值分别比TRV-CmWRKY15-1高1.99倍、2.84倍和1.39倍。PAL和CHI活性峰值分别是TRV-CmWRKY15-1的1.63倍和1.12倍。MDA和可溶性糖含量也证实，当……沉默时，菊花对病原菌更敏感。不同时间点……、……、……和……的表达水平表明，在……感染下，TRV-WRKY15-1中防御酶相关基因的表达受到抑制，削弱了菊花抵抗白锈病的能力。综上所述，……可能通过提高保护酶系统活性增强菊花对白锈病的抗性，为培育抗病新品种奠定了基础。
