Otabe Koji, Muneta Takeshi, Kawashima Nobuyuki, Suda Hideaki, Tsuji Kunikazu, Sekiya Ichiro
Orthopedic Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo , Japan.
Orthopedic Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan; †Global Center of Excellence Program for International Research Center for Molecular Science in Tooth and Bone Disease, Tokyo Medical and Dental University, Tokyo, Japan.
Cell Med. 2012 Aug 10;4(1):13-21. doi: 10.3727/215517912X653319. eCollection 2012 Jan.
The specific properties of mesenchymal stem cells (MSCs) in oral tissues still remain unknown though their existence has been previously reported. We collected gingiva, dental pulp, and periodontal ligament tissues from removed teeth and isolated MSCs. These MSCs were compared in terms of their yields per tooth, surface epitopes, and differentiation potentials by patient-matched analysis. For in vivo calcification analysis, rat gingival and dental pulp cells mounted on β-tricalcium phospateTCP were transplanted into the perivertebral muscle of rats for 6 weeks. Gingival cells and dental pulp cells showed higher yield per tooth than periodontal ligament cells (n=6, p<0.05). Yields of periodontal ligament cells were too low for further analysis. Gingival and dental pulp cells expressed MSC markers such as CD44, CD90, and CD166. Gingival and dental pulp cells obtained phenotypes of chondrocytes and adipocytes in vitro. Approximately 60% of the colonies of gingival cells and 40% of the colonies of dental pulp cells were positively stained with alizarin red in vitro, and both gingival and dental pulp cells were calcified in vivo. We clarified properties of MSCs derived from removed teeth. We could obtain a high yield of MSCs with osteogenic potential from gingiva and dental pulp. These results indicate that gingiva and dental pulp are putative cell sources for hard tissue regeneration.
尽管先前已有报道间充质干细胞（MSCs）的存在，但其在口腔组织中的具体特性仍不清楚。我们从拔除的牙齿中收集牙龈、牙髓和牙周韧带组织，并分离出间充质干细胞。通过患者匹配分析，对这些间充质干细胞在每颗牙齿的产量、表面表位和分化潜能方面进行了比较。为了进行体内钙化分析，将接种在β-磷酸三钙（TCP）上的大鼠牙龈和牙髓细胞移植到大鼠椎旁肌肉中6周。牙龈细胞和牙髓细胞每颗牙齿的产量高于牙周韧带细胞（n = 6，p < 0.05）。牙周韧带细胞的产量过低，无法进行进一步分析。牙龈和牙髓细胞表达间充质干细胞标志物，如CD44、CD90和CD166。牙龈和牙髓细胞在体外获得了软骨细胞和脂肪细胞的表型。体外约60%的牙龈细胞集落和40%的牙髓细胞集落被茜素红阳性染色，并且牙龈和牙髓细胞在体内均发生钙化。我们阐明了来源于拔除牙齿的间充质干细胞的特性。我们可以从牙龈和牙髓中获得高产率的具有成骨潜能的间充质干细胞。这些结果表明，牙龈和牙髓是硬组织再生的潜在细胞来源。
