丰富环境可逆转慢性睡眠剥夺对海马齿状回细胞可塑性造成的损伤。
Enriched environment can reverse chronic sleep deprivation-induced damage to cellular plasticity in the dentate gyrus of the hippocampus.
作者信息
Shixing Xue, Xueyan Hou, Yuan Ren, Wei Tang, Wei Wang
机构信息
Department of Neurology, Affiliated Xinhua Hospital of Dalian University, Dalian, Liaoning Province, China.
Department of Medical Imaging, Affiliated Xinhua Hospital of Dalian University, Dalian, Liaoning Province, China.
出版信息
Transl Neurosci. 2023 Mar 21;14(1):20220280. doi: 10.1515/tnsci-2022-0280. eCollection 2023 Jan 1.
OBJECTIVE
We studied whether enriched environment (EE), a classic epigenetics paradigm, can prevent cellular plasticity damage caused by chronic sleep deprivation (SD).
METHODS
We performed SD in mice by a modified multi-platform method (MMPM). Mice in the SD group were deprived of sleep for 18 h a day. In addition, half of the mice in the chronic SD group were exposed to EE stimuli for 6 h per day. Immunostaining analyzed neurogenesis and neural progenitor cell-differentiated phenotypes in the hippocampal dentate gyrus (DG) region.
RESULT
At 13 weeks, compared with the control group, SD severely impaired the proliferation and differentiation of neural stem cells, and EE completely reversed the process. SD can induce gliosis in the mouse hippocampus, and EE can delay the process.
CONCLUSION
Our results suggest that chronic SD may damage the neurogenesis in the DG of the hippocampus. However, enrichment stimulation can reverse the processing by promoting neuronal repair related to neuronal plasticity.
目的
我们研究了丰富环境（EE）这一经典的表观遗传学范式是否能够预防慢性睡眠剥夺（SD）所导致的细胞可塑性损伤。
方法
我们通过改良多平台方法（MMPM）对小鼠进行睡眠剥夺。SD组小鼠每天被剥夺睡眠18小时。此外，慢性SD组中的一半小鼠每天接受6小时的丰富环境刺激。免疫染色分析了海马齿状回（DG）区域的神经发生和神经祖细胞分化表型。
结果
在13周时，与对照组相比，睡眠剥夺严重损害了神经干细胞的增殖和分化，而丰富环境完全逆转了这一过程。睡眠剥夺可诱导小鼠海马中的胶质增生，而丰富环境可延缓这一过程。
结论
我们的结果表明，慢性睡眠剥夺可能会损害海马齿状回中的神经发生。然而，丰富环境刺激可通过促进与神经元可塑性相关的神经元修复来逆转这一过程。
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