竹笋膳食纤维可缓解高脂饮食诱导肥胖小鼠的肠道微生物群失调，并调节肝脏脂肪酸代谢。
Bamboo shoot dietary fiber alleviates gut microbiota dysbiosis and modulates liver fatty acid metabolism in mice with high-fat diet-induced obesity.
作者信息
Zhou Xiaolu, Ma Lingjun, Dong Li, Li Daotong, Chen Fang, Hu Xiaosong
机构信息
Key Laboratory of Fruits and Vegetables Processing, College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, Beijing, China.
出版信息
Front Nutr. 2023 Mar 9;10:1161698. doi: 10.3389/fnut.2023.1161698. eCollection 2023.
INTRODUCTION
Obesity is a common nutritional disorder characterized by an excessive fat accumulation. In view of the critical role of gut microbiota in the development of obesity and metabolic diseases, novel dietary therapies have been developed to manage obesity by targeting the gut microbiome. In this study, we investigated anti-obesity effects of bamboo shoot dietary fiber (BSDF) and the potential mechanisms.
METHODS
After 12 weeks of intervention with BSDF in high-fat mice, we detected obesity-related phenotypic indicators, and made transcriptomic analysis of liver tissue. Then we analyzed the changes of gut microbiota using 16S rRNA gene sequencing, explored the effect of BSDF on gut microbiota metabolites, and finally verified the importance of gut microbiota through antibiotic animal model.
RESULTS AND DISCUSSION
We found that BSDF was effective in reducing lipid accumulation in liver and adipose tissue and alleviating dyslipidemia and insulin resistance. Liver transcriptome analysis results showed that BSDF could improve lipid metabolism and liver injury by modulating peroxisome proliferator-activated receptor (PPAR) and fatty acid metabolic pathways. The 16S rRNA gene sequencing analysis of gut microbiota composition showed that BSDF significantly enriched beneficial bacteria such as , and . Analysis of fecal metabolomics and gut microbiota metabolites revealed that BSDF increased the levels of several short-chain fatty acids and enriched bile acids, which may be important for improving lipid metabolism. Notably, the obesity-related metabolic disorders were abrogated after the abrogation of gut microbiota, suggesting that gut microbiota is a key factor in the beneficial effects of BSDF.
CONCLUSION
Our study suggests that BSDF as a prebiotic supplement has the potential to improve obesity by improving gut microbiota and modulating host PPAR and fatty acid metabolic pathways.
引言
肥胖是一种常见的营养紊乱疾病，其特征是脂肪过度堆积。鉴于肠道微生物群在肥胖和代谢性疾病发展中的关键作用，人们已开发出新型饮食疗法，通过靶向肠道微生物群来控制肥胖。在本研究中，我们调查了竹笋膳食纤维（BSDF）的抗肥胖作用及其潜在机制。
方法
对高脂小鼠进行12周的BSDF干预后，我们检测了与肥胖相关的表型指标，并对肝脏组织进行了转录组分析。然后，我们使用16S rRNA基因测序分析肠道微生物群的变化，探索BSDF对肠道微生物群代谢产物的影响，最后通过抗生素动物模型验证肠道微生物群的重要性。
结果与讨论
我们发现BSDF能有效减少肝脏和脂肪组织中的脂质积累，缓解血脂异常和胰岛素抵抗。肝脏转录组分析结果表明，BSDF可通过调节过氧化物酶体增殖物激活受体（PPAR）和脂肪酸代谢途径来改善脂质代谢和肝损伤。对肠道微生物群组成的16S rRNA基因测序分析表明，BSDF显著富集了诸如 、 和 等有益细菌。粪便代谢组学和肠道微生物群代谢产物分析显示，BSDF增加了几种短链脂肪酸的水平并富集了胆汁酸，这可能对改善脂质代谢很重要。值得注意的是，肠道微生物群被消除后，与肥胖相关的代谢紊乱也被消除，这表明肠道微生物群是BSDF产生有益作用的关键因素。
结论
我们的研究表明，BSDF作为一种益生元补充剂，有潜力通过改善肠道微生物群以及调节宿主PPAR和脂肪酸代谢途径来改善肥胖。
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