Hou Congli, Tian Linyue, Lian Guoli, Fan Li-Hai, Li Zheng-Jun
College of Chemical Engineering, Fujian Engineering Research Center of Advanced Manufacturing Technology for Fine Chemicals, Fuzhou University, Fuzhou, 350108, People's Republic of China.
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Synth Syst Biotechnol. 2023 Mar 16;8(2):235-241. doi: 10.1016/j.synbio.2023.03.004. eCollection 2023 Jun.
Fumarate is a value-added chemical that is widely used in food, medicine, material, and agriculture industries. With the rising attention to the demand for fumarate and sustainable development, many novel alternative ways that can replace the traditional petrochemical routes emerged. The cell-free multi-enzyme catalysis is an effective method to produce high value chemicals. In this study, a multi-enzyme catalytic pathway comprising three enzymes for fumarate production from low-cost substrates acetate and glyoxylate was designed. The acetyl-CoA synthase, malate synthase, and fumarase from were selected and the coenzyme A achieved recyclable. The enzymatic properties and optimization of reaction system were investigated, reaching a fumarate yield of 0.34 mM with a conversion rate of 34% after 20 h of reaction. We proposed and realized the conversion of acetate and glyoxylate to fumarate using a cell-free multi-enzyme catalytic system, thus providing an alternative approach for the production of fumarate.
富马酸盐是一种具有附加值的化学品，广泛应用于食品、医药、材料和农业等行业。随着对富马酸盐需求和可持续发展关注度的不断提高，许多能够替代传统石化路线的新型方法应运而生。无细胞多酶催化是生产高价值化学品的一种有效方法。在本研究中，设计了一条由三种酶组成的多酶催化途径，用于从低成本底物乙酸盐和乙醛酸生产富马酸盐。选择了来自[具体来源未提及]的乙酰辅酶A合成酶、苹果酸合酶和富马酸酶，并实现了辅酶A的循环利用。研究了酶学性质和反应体系的优化，反应20小时后富马酸盐产量达到0.34 mM，转化率为34%。我们提出并实现了使用无细胞多酶催化系统将乙酸盐和乙醛酸转化为富马酸盐，从而为富马酸盐的生产提供了一种替代方法。
