Xu Li, Bu Chun-Qing, Kang Jie, Jia Guo-Tao, Zhang Nan, Han Fa-Bin
Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital/Liaocheng University. Liaocheng 252000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2022 Dec;31(6):581-587.
To trace the fate of transplanted stem cells from human exfoliated deciduous teeth (SHED) in the repair of periodontal bone defects, Molday ION rhodamine B (MIRB) was used to label SHED and explore the mechanism of SHED in the repair of periodontal bone defects.
In vitro cultured SHED were labeled by MIRB. The labeling efficiency, cell survival, proliferation and osteogenic differentiation of MIRB-labelled SHED were detected. The labeled cells were transplanted into the rat model with periodontal bone defect. The survival, differentiation and improvement of host periodontal bone healing of MIRB labeled SHED in vivo were analyzed by immunohistochemistry and fluorescence co-staining, nuclear magnetic imaging dual-mode tracking and H-E staining. The data were statistically analyzed with SPSS 24.0 software package.
MIRB labeled SHED did not affect its growth and osteogenic differentiation. The optimal labeling concentration was 25 μg/mL, the labeling efficiency of SHED reached 100%. The transplantation of MIRB labeled SHED in vivo can survive for more than 8 weeks. It was found that MIRB labeled SHED could differentiate into osteoblasts in vivo and significantly promote the repair of alveolar bone defects.
MIRB labeled SHED can be traced in vivo, and the effect of labeled SHED on the repair of defective alveolar bone was observed.
为追踪人脱落乳牙干细胞（SHED）在牙周骨缺损修复中的命运，采用莫尔戴异硫氰酸罗丹明B（MIRB）标记SHED，并探讨SHED在牙周骨缺损修复中的机制。
用MIRB标记体外培养的SHED。检测MIRB标记的SHED的标记效率、细胞存活、增殖和成骨分化情况。将标记细胞移植到牙周骨缺损大鼠模型中。通过免疫组织化学和荧光共染色、核磁共振成像双模态追踪及苏木精-伊红染色分析MIRB标记的SHED在体内的存活、分化及对宿主牙周骨愈合的改善情况。数据用SPSS 24.0软件包进行统计学分析。
MIRB标记SHED不影响其生长和成骨分化。最佳标记浓度为25μg/mL，SHED的标记效率达100%。MIRB标记的SHED在体内移植后可存活8周以上。发现MIRB标记的SHED在体内可分化为成骨细胞，并显著促进牙槽骨缺损的修复。
MIRB标记的SHED在体内可被追踪，且观察到标记的SHED对缺损牙槽骨修复的作用。
