Grob U, Stüber K
Max-Planck-Institut für Immunbiologie, Frieburg, FRG.
Nucleic Acids Res. 1987 Dec 10;15(23):9957-73. doi: 10.1093/nar/15.23.9957.
We aligned 14 5'-leading sequences of small subunit ribulose-1,5-bisphosphate carboxylase (rbcS) genes. A strong consensus sequence ("CCTTATCAT") was located directly upstream of the TATA-box. The occurrence of this motif in other light dependent phytochrome regulated plant genes led to the calculation of two consensus matrices. With these two matrices we are able to distinguish almost all known light induced plant genes which are phytochrome regulated from non-light induced plant genes indicating, that all these genes share a common light-responsive element (LRE). The results obtained by computer analysis are discussed with regard to experimental data.
我们比对了14个小亚基核酮糖-1,5-二磷酸羧化酶(rbcS)基因的5'端前导序列。一个强共有序列(“CCTTATCAT”)直接位于TATA框的上游。该基序在其他光依赖型光敏色素调节的植物基因中的出现,促使我们计算了两个共有矩阵。利用这两个矩阵,我们几乎能够区分所有已知的受光敏色素调节的光诱导植物基因与非光诱导植物基因,这表明所有这些基因共享一个共同的光响应元件(LRE)。结合实验数据对计算机分析所得结果进行了讨论。