Tocík Z, Arnold L, Smrt J
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Praha.
Nucleic Acids Symp Ser. 1987(18):193-6.
Side reaction during phosphitylation of 5'-O-dimethoxytrityl-N2-isobutyryl-2'-deoxyguanosine was observed. As a suitable dG synthon, 5'-O-dimethoxytrityl-N2-dimethylaminomethyl-ene-3'-H-phosphonate was developed and used, along with 3'-H-phosphonates of 5'-O-dimethoxytrityl derivatives of dT, N4--benzoyl-dC and N6-dimethylaminoethylidene-dA, in automated synthesis of several oligodeoxyribonucleotides containing 30-40 bases.
观察到5'-O-二甲氧基三苯甲基-N2-异丁酰基-2'-脱氧鸟苷磷酸化过程中的副反应。作为一种合适的dG合成子，开发并使用了5'-O-二甲氧基三苯甲基-N2-二甲基氨基亚甲基-3'-H-膦酸酯，与dT、N4-苯甲酰基-dC和N6-二甲基氨基亚乙基-dA的5'-O-二甲氧基三苯甲基衍生物的3'-H-膦酸酯一起，用于自动合成几种含30 - 40个碱基的寡脱氧核糖核苷酸。
