Li Li, Ma Xiaoxie, Peng Yusong, Yin Jun, Guissi Nida El Islem, Wang Yiqing
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210023, People's Republic of China.
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China.
ACS Appl Bio Mater. 2023 Apr 17;6(4):1639-1649. doi: 10.1021/acsabm.3c00080. Epub 2023 Mar 27.
Fluorescent probes with fluorescence emission in the NIR-II window have been widely studied due to increased imaging depth. However, the currently reported NIR-II fluorescent probes present some disadvantages, such as complicated synthesis routes and low fluorescence quantum yields (QYs). The shielding strategy has been used in the development of NIR-II probes to improve their QYs. So far, this strategy has only been used for the symmetric NIR-II probes, especially those based on the benzo[1,2-c:4,5-c']bis([1,2,5]thiadiazole) (BBTD) skeleton. This work reports the synthesis of a series of asymmetric NIR-II probes based on shielding strategies accompanied by simple synthetic routes, high synthetic yields (above 90%), high QYs, and large Stoke shifts. Furthermore, the use of d-α-tocopheryl polyethylene glycol succinate (TPGS) as a surfactant for an NIR-II fluorescence probe (NT-4) improved its water solubility. In vivo studies showed that TPGS-NT-4 NPs with a high QY (3.46%) achieve high-resolution angiography and efficient local photothermal therapy, while displaying good biocompatibility. Hence, we combined angiography and local photothermal therapy to improve the tumor uptake of nanophotothermal agents while reducing their damage to normal tissues.
由于成像深度增加，在近红外二区窗口发射荧光的荧光探针已得到广泛研究。然而，目前报道的近红外二区荧光探针存在一些缺点，如合成路线复杂和荧光量子产率（QYs）低。屏蔽策略已被用于近红外二区探针的开发以提高其量子产率。到目前为止，该策略仅用于对称近红外二区探针，特别是那些基于苯并[1,2-c:4,5-c']双（[1,2,5]噻二唑）（BBTD）骨架的探针。本文报道了一系列基于屏蔽策略的不对称近红外二区探针的合成，其具有简单的合成路线、高合成产率（高于90%）、高量子产率和大斯托克斯位移。此外，使用d-α-生育酚聚乙二醇琥珀酸酯（TPGS）作为近红外二区荧光探针（NT-4）的表面活性剂提高了其水溶性。体内研究表明，具有高量子产率（3.46%）的TPGS-NT-4纳米粒子实现了高分辨率血管造影和高效的局部光热治疗，同时显示出良好的生物相容性。因此，我们将血管造影和局部光热治疗相结合，以提高纳米光热剂在肿瘤中的摄取，同时减少其对正常组织的损伤。
