Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, People's Republic of China.
Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, People's Republic of China.
Mikrochim Acta. 2023 Mar 27;190(4):157. doi: 10.1007/s00604-023-05750-x.
A fluorescence and colorimetric tandem dual-mode sensor was established by modulating fluorescence and oxidase-like activity via valence switching of cerium-based coordination polymer nanoparticles (Ce-CPNs) for the detection of sarcosine (Sar) which is considered as a potential biomarker for the diagnosis of prostate cancer (PCa). In the present research, sarcosine oxidase (SOX) specifically catalyzes the oxidation of Sar to yield HO which can rapidly oxidize Ce(III)-CPNs to generate Ce(IV)-CPNs in appropriate alkaline solution. The generated Ce(IV)-CPNs create a markedly weakened fluorescent signal at 350 nm, while they can induce oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to generate blue TMBox through emerging good oxidase-like activity. The sensing platform can realize accurate, stable, and high-throughput detection of Sar because of the tandem dual signal output mechanism. More attractively, the chromogenic hydrogel sensing device using smartphone photographing has achieved perfect results for the on-site sensing of Sar in urine specimens without large experimental equipments, demonstrating its considerable clinical application potential in the early diagnosis of PCa.
建立了一种荧光和比色串联双重模式传感器，通过调节基于铈的配位聚合物纳米粒子（Ce-CPNs）的价态转换来实现荧光和氧化酶样活性，用于检测肌氨酸（Sar），Sar 被认为是前列腺癌（PCa）诊断的潜在生物标志物。在本研究中，肌氨酸氧化酶（SOX）特异性地催化 Sar 的氧化，生成 HO，HO 可以在适当的碱性溶液中快速地将 Ce(III)-CPNs 氧化为 Ce(IV)-CPNs。生成的 Ce(IV)-CPNs 在 350nm 处产生明显减弱的荧光信号，同时由于新出现的良好氧化酶样活性，可以将 3,3',5,5'-四甲基联苯胺（TMB）氧化生成蓝色 TMBox。由于串联双重信号输出机制，该传感平台可以实现 Sar 的准确、稳定和高通量检测。更吸引人的是，使用智能手机拍照的比色水凝胶传感装置已经实现了尿液样本中 Sar 的现场传感的完美结果，而无需大型实验设备，显示出其在 PCa 早期诊断中的相当大的临床应用潜力。
