College of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China; School of Basic Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China.
College of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China.
Anal Chim Acta. 2022 Sep 22;1227:340308. doi: 10.1016/j.aca.2022.340308. Epub 2022 Aug 26.
Herein, a colorimetric sensing system based on cerium(IV) coordination polymer nanoparticles (Ce(IV)-ATP-Tris CPNs) was proposed for As(V) detection. Ce(IV)-ATP-Tris CPNs show excellent oxidase-like activity, triggering 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic reaction. With addition of ascorbic acid 2-phosphate (AAP) and acid phosphatase (ACP), ACP can hydrolyze AAP to produce antioxidative ascorbic acid (AA), inhibiting TMB chromogenic reaction. After that, introduction of As(V) can inhibit ACP, recovering TMB chromogenic reaction. Therefore, sensitive and selective As(V) detection is achieved. Moreover, Ce(IV)-ATP-Tris CPNs were transformed into cellulose nanofiber (CNF) to form test strip (Ce(IV)-ATP-Tris CPNs/CNF). Inorganic arsenic in rice can be detected by test strip, color of that can be measured by smartphone-integrated colorimetric quantitative analysis platform. Given this, rapid and convenient strip test of inorganic arsenic in rice by using this platform was achieved. Hence, this platform possesses great application potential in the field of inorganic arsenic in rice, even food safety.
本文提出了一种基于铈（IV）配位聚合物纳米粒子（Ce（IV）-ATP-Tris CPNs）的比色传感系统，用于检测 As(V)。Ce（IV）-ATP-Tris CPNs 具有优异的过氧化物酶样活性，可引发 3,3',5,5'-四甲基联苯胺（TMB）显色反应。加入抗坏血酸 2-磷酸酯（AAP）和酸性磷酸酶（ACP）后，ACP 可将 AAP 水解生成抗氧化性抗坏血酸（AA），从而抑制 TMB 显色反应。随后，引入的 As(V)可抑制 ACP，恢复 TMB 显色反应。因此，实现了对 As(V)的灵敏和选择性检测。此外，Ce(IV)-ATP-Tris CPNs 被转化为纤维素纳米纤维（CNF），形成测试条（Ce(IV)-ATP-Tris CPNs/CNF）。该测试条可用于检测大米中的无机砷，其颜色可通过智能手机集成的比色定量分析平台进行测量。有鉴于此，通过使用该平台实现了大米中无机砷的快速便捷的条带测试。因此，该平台在大米无机砷甚至食品安全领域具有巨大的应用潜力。
