Faculdade de Ciências Agronômicas, Laboratório de Controle Biológico de Pragas Florestais, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brasil.
Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil.
PLoS One. 2023 Mar 27;18(3):e0283543. doi: 10.1371/journal.pone.0283543. eCollection 2023.
Insect pests introduced in eucalyptus plantations in Brazil are mostly of Australian origin, but native microorganisms have potential for their management. High quality biopesticide production based on entomopathogenic fungi depends on adequate technologies. The objective of this study was to evaluate Mycoharvester® equipment to harvest and separating particles to obtain pure Metarhizium anisopliae conidia to manage Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Hemiptera: Thaumastocoridae). The Mycoharvester® version 5b harvested and separated M. anisopliae spores. The pure conidia were suspended in Tween 80® (0.1%) and calibrated to the concentrations of 1 x 106, 107, 108 and 109 conidia/ml to evaluate the pathogenicity, lethal concentration 50 and 90 (LC50, LC90) and lethal time 50 and 90 (LT50, LT90) of this fungus to T. peregrinus. This equipment harvested 85% of the conidia from rice, with production of 4.8 ± 0.38 x 109 conidia/g dry mass of substrate + fungus. The water content of 6.36% of the single spore powder (pure conidia) separated by the Mycoharvester® was lower than that of the agglomerated product. The product harvested at the concentrations of 108 and 109 conidia/ml caused high mortality to T. peregrinus third instar nymphs and adults. The separation of conidia produced by solid-state fermentation with the Mycoharvester® is an important step toward optimizing the fungal production system of pure conidia, and to formulate biopesticides for insect pest management.
巴西桉树种植园中引入的害虫主要来自澳大利亚，但本地微生物对其管理具有潜力。基于昆虫病原真菌的高质量生物农药生产依赖于适当的技术。本研究的目的是评估 Mycoharvester®设备，以收获和分离颗粒，获得纯绿僵菌分生孢子，以管理 Thaumastocoris peregrinus Carpintero & Dellapé, 2006（半翅目：异蝽科）。Mycoharvester®版本 5b 收获和分离绿僵菌孢子。纯分生孢子悬浮在 Tween 80®（0.1%）中，并校准至浓度为 1 x 106、107、108 和 109 个分生孢子/ml，以评估该真菌对 T. peregrinus 的致病性、半数致死浓度 50 和 90（LC50、LC90）和致死时间 50 和 90（LT50、LT90）。该设备从水稻中收获了 85%的分生孢子，生产了 4.8 ± 0.38 x 109 个分生孢子/g 干质量的基质+真菌。Mycoharvester®分离的单孢子粉（纯分生孢子）的含水量为 6.36%，低于团聚产品。在浓度为 108 和 109 个分生孢子/ml 下收获的产品对 T. peregrinus 三龄若虫和成虫的致死率很高。用 Mycoharvester®进行固态发酵生产的分生孢子的分离是优化纯分生孢子真菌生产系统和制定用于害虫管理的生物农药的重要步骤。
