Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, P. R. China.
Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325006, P. R. China.
Adv Sci (Weinh). 2023 May;10(15):e2207438. doi: 10.1002/advs.202207438. Epub 2023 Mar 27.
The monitoring of tissue regeneration is particularly important. However, most materials do not allow direct observation of the regeneration process in the cartilage layer. Here, using sulfhydryl polyhedral oligomeric silsesquioxane (POSS-SH) as a nano-construction platform, poly(ethylene glycol) (PEG), Kartogenin (KGN), hydrogenated soya phosphatidylcholine (HSPC), and fluorescein are linked through the "click chemistry" method to construct nanomaterial with fluorescence visualization for cartilage repair: POSS linked with PEG, KGN, HSPC, and fluorescein (PPKHF). PPKHF nanoparticles are encapsulated with hyaluronic acid methacryloyl to prepare PPKHF-loaded microfluidic hyaluronic acid methacrylate spheres (MHS@PPKHF) for in situ injection into the joint cavity using microfluidic technology. MHS@PPKHF forms a buffer lubricant layer in the joint space to reduce friction between articular cartilages, while releasing encapsulated positively charged PPKHF to the deep cartilage through electromagnetic force, facilitating visualization of the location of the drug via fluorescence. Moreover, PPKHF facilitates differentiation of bone marrow mesenchymal stem cells into chondrocytes, which are located in the subchondral bone. In animal experiment, the material accelerates cartilage regeneration while allowing monitoring of cartilage layer repair progression via fluorescence signals. Thus, these POSS-based micro-nano hydrogel microspheres can be used for cartilage regeneration and monitoring and potentially for clinical osteoarthritis therapy.
组织再生的监测尤为重要。然而，大多数材料不允许直接观察软骨层的再生过程。在这里，我们使用巯基笼型倍半硅氧烷（POSS-SH）作为纳米结构平台，通过“点击化学”方法将聚乙二醇（PEG）、卡托金（KGN）、氢化大豆卵磷脂（HSPC）和荧光素连接起来，构建具有荧光可视化功能的用于软骨修复的纳米材料：巯基笼型倍半硅氧烷连接的聚乙二醇、卡托金、氢化大豆卵磷脂和荧光素（PPKHF）。将 PPKHF 纳米颗粒包封在透明质酸甲基丙烯酰中，以制备负载 PPKHF 的微流控透明质酸甲基丙烯酰球体（MHS@PPKHF），并使用微流控技术原位注射到关节腔中。MHS@PPKHF 在关节腔内形成缓冲润滑层，减少关节软骨之间的摩擦，同时通过电磁力将包封的带正电荷的 PPKHF 释放到深层软骨中，通过荧光便于药物位置的可视化。此外，PPKHF 促进骨髓间充质干细胞向位于软骨下骨的软骨细胞分化。在动物实验中，该材料通过荧光信号加速软骨再生，同时允许监测软骨层修复进展。因此，这些基于 POSS 的微纳米水凝胶微球可用于软骨再生和监测，并有可能用于临床骨关节炎治疗。
