Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, 400076, India.
Macromol Biosci. 2024 Jan;24(1):e2200562. doi: 10.1002/mabi.202200562. Epub 2023 Apr 7.
Scaffold-based cell delivery can improve therapeutic effects of transplanted cells in cell therapy. Biomaterial scaffolds serveas niche for cell growth and proliferation which improves cell survival and overall function post cell delivery. In this study, gelatin methacryloyl based injectable scaffolds made using poly(ethylene)glycol as a sacrificial polymer and cryogelation as a technique, are demonstrated to have tunable degradability and porosity that is required for cell and drug delivery applications. The pore size (10-142 µm) of these gels makes them suitable for loading different cell types as per the application. In vitro studies using mammalian cells confirm that these cryogels are cytocompatible. These cell-laden scaffolds are injectable and have a cell retention ability of up to 90% after injection. Rheology is done to evaluate stiffness and shape recovery property, and it is found that these gels can maintain their original shape even after applying 7 cycles of strain from 0.1% to 20%. Furthermore, their degradability can be modulated between 6 and 10 days by changing the overall polymer composition. Thus, injectability and degradability of these cryogels can circumvent invasive surgical procedures, thereby making them useful for a variety of applications including delivery of cells and bioactive factors.
支架细胞递送可以提高细胞治疗中转基因细胞的治疗效果。生物材料支架作为细胞生长和增殖的小生境，提高了细胞的存活率和细胞递送后的整体功能。在这项研究中，使用聚乙二醇作为牺牲聚合物和冷冻凝胶化作为技术的基于明胶甲基丙烯酰的可注射支架，被证明具有可调节的降解性和孔隙率，这是细胞和药物递送应用所必需的。这些凝胶的孔径（10-142µm）使其适合根据应用加载不同类型的细胞。使用哺乳动物细胞的体外研究证实这些冷冻凝胶具有细胞相容性。这些负载细胞的支架是可注射的，并且在注射后具有高达 90%的细胞保留能力。流变学用于评估硬度和形状恢复特性，结果发现，即使在从 0.1%到 20%的应变施加 7 个循环后，这些凝胶仍能保持其原始形状。此外，通过改变整体聚合物组成，可以将其降解性在 6 到 10 天之间进行调节。因此，这些冷冻凝胶的可注射性和可降解性可以避免侵入性手术程序，从而使其适用于各种应用，包括细胞和生物活性因子的递送。
