Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Pharmacology and Therapy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Asian Pac J Cancer Prev. 2023 Mar 1;24(3):1095-1099. doi: 10.31557/APJCP.2023.24.3.1095.
This study aimed to explore the diagnostic performance of miRNA-21 to differentiate between Prostate Cancer (PCa) and Benign Prostatic Hyperplasia (BPH) patients in Indonesia.
Urine samples were collected from each PCa and BPH patient. miRNA-21 relative expression against the reference gene was analyzed and compared between the two. miRNA expression was then analyzed using the comparative quantification method to find the fold change. miR-21 validity in identifying PCa patients was performed by quantifying the sensitivity and specificity using samples in this study.
The results of this study indicated that miRNA-21 relative expression against miRNA-16 in PCa and BPH showed 12.95 differences in fold change. Moreover, using prostate biopsy as the gold standard to differentiate PCa and BPH, miRNA-21 Cq expression has 100% sensitivity and 75% specificity in differentiating the two.
miRNA-21 relative expression can be used to discriminate PCa from BPH by using a urine sample. Furthermore, the expression of miR-21 has higher sensitivity than PSA; therefore, miR-21 has a high potential to be analyzed and developed further for clinical diagnosis of prostate cancer.
本研究旨在探索 microRNA-21（miRNA-21）在印度尼西亚前列腺癌（PCa）和良性前列腺增生（BPH）患者中的诊断性能。
从每位 PCa 和 BPH 患者中采集尿液样本。分析并比较了两种疾病患者的 miRNA-21 与参照基因的相对表达。然后使用比较定量法分析 miRNA 表达，以找到倍数变化。使用本研究中的样本，通过定量分析敏感性和特异性来评估 miR-21 对识别 PCa 患者的有效性。
本研究结果表明，PCa 和 BPH 患者的 miRNA-21 与 miRNA-16 的相对表达在倍数变化上存在 12.95 的差异。此外，使用前列腺活检作为区分 PCa 和 BPH 的金标准，miRNA-21 的 Cq 表达在区分两者方面具有 100%的敏感性和 75%的特异性。
miRNA-21 的相对表达可用于通过尿液样本区分 PCa 和 BPH。此外，miR-21 的表达比 PSA 具有更高的敏感性；因此，miR-21 具有很高的分析和进一步开发用于前列腺癌临床诊断的潜力。
