hsa-mir-106b-5p 在印度尼西亚前列腺癌患者液体生物标志物中的潜力。
The Potential of hsa-mir-106b-5p as Liquid Biomarker in Prostate Cancer Patients in Indonesia.
机构信息
Department of Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Biomedical Science., Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia.
出版信息
Asian Pac J Cancer Prev. 2021 Mar 1;22(3):837-842. doi: 10.31557/APJCP.2021.22.3.837.
PURPOSE
This study aims to explore the potential of hsa-mir-106b-5p as a new liquid biomarker for prostate cancer sufferers in Indonesia.
METHODS
Analysis of hsa-mir-106b-5p expression of two tissue samples from BPH patients and two PCa patients used NanoString nCounter Expression Assay then validated by qRT-PCR using 10 patient urine samples for prostate cancer and BPH. Furthermore, analysis of the role of hsa-mir-106b-5p in prostate cancer was carried out bioinformatically.
RESULTS
The results of this study indicated that the expression of hsa-mir-106b-5p in prostate cancer tissue was 1.23 times higher than that of BPH and urine of Indonesian patients (1.72 times). Moreover, this miRNA was upregulated in prostate cancer cells compared to normal cells 1.37 times. The hsa-mir-106b-5p appeared to be involved in the development of prostate cancer through the binding of genes involved in endoplasmic reticulum stress pathways and tumor suppressor genes.
CONCLUSION
hsa-mir-106b-5p could modulate prostate cancer by interfering with the endoplasmic reticulum stress repair pathways and decreasing the expression of tumor suppressor genes involved in many biological processes. These updates our understanding of the role of hsa-mir-106b-5p in cancer and its potential as a candidate of a biomarker for clinical diagnosis of prostate cancer.
目的
本研究旨在探索 hsa-mir-106b-5p 作为印度尼西亚前列腺癌患者新型液体生物标志物的潜力。
方法
使用 NanoString nCounter Expression Assay 分析 2 例 BPH 患者和 2 例 PCa 患者组织样本中 hsa-mir-106b-5p 的表达情况，并用 10 例前列腺癌和 BPH 患者的尿液样本进行 qRT-PCR 进行验证。此外，还通过生物信息学分析了 hsa-mir-106b-5p 在前列腺癌中的作用。
结果
本研究结果表明，前列腺癌组织中 hsa-mir-106b-5p 的表达水平比 BPH 和印度尼西亚患者的尿液高 1.23 倍（1.72 倍）。此外，该 miRNA 在前列腺癌细胞中的表达水平比正常细胞高 1.37 倍。hsa-mir-106b-5p 似乎通过与内质网应激途径和肿瘤抑制基因相关的基因结合，参与了前列腺癌的发生发展。
结论
hsa-mir-106b-5p 可能通过干扰内质网应激修复途径和降低参与许多生物过程的肿瘤抑制基因的表达，从而调节前列腺癌。这些发现更新了我们对 hsa-mir-106b-5p 在癌症中的作用及其作为前列腺癌临床诊断生物标志物候选物的潜力的认识。
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