Fadeeva Irina S, Teterina Anastasia Yu, Minaychev Vladislav V, Senotov Anatoliy S, Smirnov Igor V, Fadeev Roman S, Smirnova Polina V, Menukhov Vladislav O, Lomovskaya Yana V, Akatov Vladimir S, Barinov Sergey M, Komlev Vladimir S
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia.
Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, Moscow 117334, Russia.
Biomimetics (Basel). 2023 Feb 23;8(1):91. doi: 10.3390/biomimetics8010091.
Bone grafts with a high potential for osseointegration, capable of providing a complete and effective regeneration of bone tissue, remain an urgent and unresolved issue. The presented work proposes an approach to develop composite biomimetic bone material for reconstructive surgery by deposition (remineralization) on the surface of high-purity, demineralized bone collagen matrix calcium phosphate layers. Histological and elemental analysis have shown reproduction of the bone tissue matrix architectonics, and a high-purity degree of the obtained collagen scaffolds; the cell culture and confocal microscopy have demonstrated a high biocompatibility of the materials obtained. Adsorption spectroscopy, scanning electron microscopy, microcomputed tomography (microCT) and infrared spectroscopy, and X-ray diffraction have proven the efficiency of the deposition of calcium phosphates on the surface of bone collagen scaffolds. Cell culture and confocal microscopy methods have shown high biocompatibility of both demineralized and remineralized bone matrices. In the model of heterotopic implantation in rats, at the term of seven weeks, an intensive intratrabecular infiltration of calcium phosphate precipitates, and a pronounced synthetic activity of osteoblast remodeling and rebuilding implanted materials, were revealed in remineralized bone collagen matrices in contrast to demineralized ones. Thus, remineralization of highly purified demineralized bone matrices significantly enhanced their osteostimulating ability. The data obtained are of interest for the creation of new highly effective osteoplastic materials for bone tissue regeneration and augmentation.
具有高骨整合潜力、能够实现骨组织完全有效再生的骨移植材料，仍然是一个亟待解决的问题。本文提出了一种通过在高纯度脱矿骨胶原基质表面沉积（再矿化）磷酸钙层来开发用于重建手术的复合仿生骨材料的方法。组织学和元素分析表明，骨组织基质结构得以重现，且所获得的胶原支架纯度很高；细胞培养和共聚焦显微镜检查表明所获得的材料具有高生物相容性。吸附光谱、扫描电子显微镜、微计算机断层扫描（microCT）、红外光谱以及X射线衍射证明了磷酸钙在骨胶原支架表面沉积的有效性。细胞培养和共聚焦显微镜检查方法表明脱矿化和再矿化骨基质均具有高生物相容性。在大鼠异位植入模型中，在七周时，与脱矿化骨胶原基质相比，再矿化骨胶原基质中出现了磷酸钙沉淀物的强烈小梁内浸润，以及成骨细胞重塑和重建植入材料的明显合成活性。因此，高纯度脱矿化骨基质的再矿化显著增强了其骨刺激能力。所获得的数据对于创建用于骨组织再生和增强的新型高效骨整形材料具有重要意义。
