Bile Carriage of -Positive Enterococcus faecium in a Patient with Choledocholith.

We isolated one Enterococcus faecium isolate SZ21B15 from a bile sample of a patient with choledocholith in Shenzhen, China in 2021. It was positive for oxazolidinone resistance gene and was intermediate to linezolid. The whole genome of E. faecium SZ21B15 was sequenced by Illumina Hiseq. It belonged to ST533 within the clonal complex 17. The gene and additional two resistance genes and (A) were located within a 25,777-bp multiresistance region, which was inserted into the chromosomal gene, being chromosomal intrinsic resistance genes. The chromosomal gene cluster found in E. faecium SZ21B15 was closely related to the corresponding regions of multiple -carrying plasmids or chromosomes from , , Staphylococcus, and strains. It further highlights the ability of the cluster that transfers between plasmids and chromosomes and evolves by a series of molecular recombination events. Oxazolidinone are effective antimicrobial agents for the treatment of infections caused by multidrug-resistant Gram-positive bacteria, including vancomycin-resistant enterococci. The emergence and global spread of transferable oxazolidinone resistance genes such as is worrisome. spp. can become causes of hospital-associated infections and are also widely distributed in the gastrointestinal tracts of animals and the natural environment. In this study, one E. faecium isolate from bile sample carried chromosomal , being intrinsic resistance gene. -positive E. faecium in bile not only makes the treatment of gallstones difficult, but also may become a reservoir of resistance genes in the body.