Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Brazil.
Department of Fisiotherapy, Federal University of São Carlos (UFSCar), São Carlos, Brazil.
J Bone Miner Metab. 2020 Sep;38(5):639-647. doi: 10.1007/s00774-020-01102-4. Epub 2020 Apr 18.
Collagen from marine esponges has been used as a promising material for tissue engineering proposals. Similarly, photobiomodulation (PBM) is able of modulating inflammatory processes after an injury, accelerating soft and hard tissue healing and stimulating neoangiogenesis. However, the effects of the associated treatments on bone tissue healing have not been studied yet. In this context, the present study aimed to evaluate the biological temporal modifications (using two experimental periods) of marine sponge collagen or sponging (SPG) based scaffold and PBM on newly formed bone using a calvaria bone defect model.
Wistar rats were distributed into two groups: SPG or SPG/PBM and euthanized into two different experimental periods (15 and 45 days post-surgery). A cranial critical bone defect was used to evaluate the effects of the treatments. Histology, histomorfometry and immunohistological analysis were performed.
Histological findings demonstrated that SPG/PBM-treated animals, 45 days post-surgery, demonstrated a higher amount of connective and newly formed bone tissue at the region of the defect compared to CG. Notwithstanding, no difference among groups were observed in the histomorphometry. Interestingly, for both anti-transforming growth factor-beta (TGF-β) and anti-vascular endothelial growth factor (VEGF) immunostaining, higher values for SPG/PBM, at 45 days post-surgery could be observed.
It can be concluded that the associated treatment can be considered as a promising therapeutical intervention.
海洋海绵中的胶原蛋白已被用作组织工程方案的有前途的材料。同样，光生物调节（PBM）能够调节损伤后的炎症过程，加速软组织和硬组织的愈合，并刺激新血管生成。然而，相关治疗对骨组织愈合的影响尚未得到研究。在这种情况下，本研究旨在使用颅骨骨缺损模型评估海洋海绵胶原蛋白或海绵（SPG）基支架和 PBM 对新形成骨的生物学时间修饰（使用两个实验期）。
Wistar 大鼠分为两组：SPG 或 SPG/PBM，并在两个不同的实验期（手术后 15 天和 45 天）处死。采用颅临界骨缺损来评估治疗效果。进行组织学、组织形态计量学和免疫组织化学分析。
组织学发现表明，与 CG 相比，在手术后 45 天，接受 SPG/PBM 治疗的动物在缺损区域显示出更多的结缔组织和新形成的骨组织。然而，在组织形态计量学上，各组之间没有观察到差异。有趣的是，对于抗转化生长因子-β（TGF-β）和抗血管内皮生长因子（VEGF）免疫染色，在手术后 45 天，SPG/PBM 的值更高。
可以得出结论，联合治疗可以被认为是一种有前途的治疗干预措施。
