Farnezi Bassi Ana Paula, Bizelli Vinícius Ferreira, Brasil Leticia Freitas de Mendes, Pereira Járede Carvalho, Al-Sharani Hesham Mohammed, Momesso Gustavo Antonio Correa, Faverani Leonardo P, Lucas Flavia de Almeida
Department of Diagnosis and Surgery, São Paulo State University, UNESP, School of Dentistry, Araçatuba, São Paulo 16015-050, Brazil.
Department of Oral and Maxillofacial Surgery, College of Dentistry, Ibb University, Ibb 16015-050, Yemen.
Membranes (Basel). 2020 Sep 12;10(9):230. doi: 10.3390/membranes10090230.
Guided bone regeneration was studied to establish protocols and develop new biomaterials that revealed satisfactory results. The present study aimed to comparatively evaluate the efficiency of the bacterial cellulose membrane (Nanoskin) and collagen membrane Bio-Gide in the bone repair of 8-mm critical size defects in rat calvaria. Seventy-two adult male rats were divided into three experimental groups (n = 24): the CG-membrane-free control group (only blood clot, negative control), BG-porcine collagen membrane group (Bio-Guide, positive control), and BC-bacterial cellulose membrane group (experimental group). The comparison periods were 7, 15, 30, and 60 days postoperatively. Histological, histometric, and immunohistochemical analyses were performed. The quantitative data were subjected to 2-way ANOVA and Tukey's post-test, and < 0.05 was considered significant. At 30 and 60 days postoperatively, the BG group showed more healing of the surgical wound than the other groups, with a high amount of newly formed bone ( < 0.001), while the BC group showed mature connective tissue filling the defect. The inflammatory cell count at postoperative days 7 and 15 was higher in the BC group than in the BG group (Tukey's test, = 0.006). At postoperative days 30 and 60, the area of new bone formed was greater in the BG group than in the other groups ( < 0.001). Immunohistochemical analysis showed moderate and intense immunolabeling of osteocalcin and osteopontin at postoperative day 60 in the BG and BC groups. Thus, despite the promising application of the BC membrane in soft-tissue repair, it did not induce bone repair in rat calvaria.
研究引导骨再生以建立方案并开发出显示出满意结果的新型生物材料。本研究旨在比较评估细菌纤维素膜（纳米皮肤）和胶原膜Bio-Gide在大鼠颅骨8毫米临界尺寸缺损骨修复中的效率。72只成年雄性大鼠被分为三个实验组（n = 24）：CG-无膜对照组（仅血凝块，阴性对照）、BG-猪胶原膜组（Bio-Guide，阳性对照）和BC-细菌纤维素膜组（实验组）。比较时期为术后7、15、30和60天。进行了组织学、组织计量学和免疫组织化学分析。定量数据进行双向方差分析和Tukey事后检验，P<0.05被认为具有显著性。术后30天和60天，BG组手术伤口的愈合情况比其他组更好，有大量新形成的骨组织（P<0.001），而BC组显示缺损处被成熟结缔组织填充。术后第7天和15天，BC组的炎性细胞计数高于BG组（Tukey检验，P = 0.006）。术后30天和60天，BG组新形成的骨组织面积大于其他组（P<0.001）。免疫组织化学分析显示，术后第60天，BG组和BC组骨钙素和骨桥蛋白的免疫标记呈中度和强阳性。因此，尽管BC膜在软组织修复中有很有前景的应用，但它并未在大鼠颅骨中诱导骨修复。
