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间歇性暴露于16赫兹极低频脉冲电磁场通过激活骨祖细胞中Piezo 1诱导的钙内流促进体外成骨。

Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca Influx in Osteoprogenitor Cells.

作者信息

Chen Yangmengfan, Braun Benedikt J, Menger Maximilian M, Ronniger Michael, Falldorf Karsten, Histing Tina, Nussler Andreas K, Ehnert Sabrina

机构信息

Siegfried Weller Institute at the BG Trauma Center Tübingen, Department of Trauma and Reconstructive Surgery, University of Tübingen, Schnarrenbergstr. 95, D-72076 Tübingen, Germany.

Sachtleben GmbH, Haus Spectrum am UKE, Martinistraße 64, D-20251 Hamburg, Germany.

出版信息

J Funct Biomater. 2023 Mar 20;14(3):165. doi: 10.3390/jfb14030165.

DOI:10.3390/jfb14030165
PMID:36976089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10055851/
Abstract

Exposure to extremely low frequency pulsed electromagnetic fields (ELF-PEMF) is supposed to simulate local EMF generated during mechanical stimulation of bone and may therefore be used to improve bone regeneration. This study aimed at optimizing the exposure strategy and investigating the underlying mechanisms of a 16 Hz ELF-PEMF, previously reported to boost osteoblast function. Comparing influences of daily continuous (30 min every 24 h) and intermittent (10 min every 8 h) exposure to the 16 Hz ELF-PEMF on osteoprogenitor cells revealed that the intermittent exposure strategy enhanced the 16 Hz ELF-PEMF effects regarding cell numbers and osteogenic function. Gene expression of and related Ca influx were significantly increased in SCP-1 cells with the daily intermittent exposure. Pharmacological inhibition of piezo 1 with Dooku 1 largely abolished the positive effect of the 16 Hz ELF-PEMF exposure on osteogenic maturation of SCP-1 cells. In summary, the intermittent exposure strategy enhanced the positive effects of 16 Hz continuous ELF-PEMF exposure in terms of cell viability and osteogenesis. This effect was shown to be mediated by an increased expression of and related Ca influx. Thus, the intermittent exposure strategy is a promising way to further optimize the therapeutic effects of the 16 Hz ELF-PEMF regarding fracture healing or osteoporosis.

摘要

暴露于极低频脉冲电磁场（ELF-PEMF）被认为可模拟骨骼机械刺激过程中产生的局部电磁场，因此可用于促进骨再生。本研究旨在优化暴露策略，并探究16 Hz ELF-PEMF的潜在机制，此前有报道称该频率的ELF-PEMF可增强成骨细胞功能。比较每日连续（每24小时30分钟）和间歇性（每8小时10分钟）暴露于16 Hz ELF-PEMF对骨祖细胞的影响，结果显示间歇性暴露策略在细胞数量和成骨功能方面增强了16 Hz ELF-PEMF的作用。每日间歇性暴露后，SCP-1细胞中Piezo1及相关钙内流的基因表达显著增加。用Dooku 1对Piezo1进行药理学抑制，很大程度上消除了16 Hz ELF-PEMF暴露对SCP-1细胞成骨成熟的积极作用。总之，间歇性暴露策略在细胞活力和成骨方面增强了16 Hz连续ELF-PEMF暴露的积极作用。这种作用被证明是由Piezo1表达增加及相关钙内流介导的。因此，间歇性暴露策略是进一步优化16 Hz ELF-PEMF在骨折愈合或骨质疏松症治疗效果方面的一种有前景的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/7c152ff3c9b1/jfb-14-00165-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/97b89cc24f95/jfb-14-00165-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/d8feca409d34/jfb-14-00165-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/c45bd1fa000f/jfb-14-00165-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/052ec9de7d36/jfb-14-00165-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/7c96023c370e/jfb-14-00165-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/7c152ff3c9b1/jfb-14-00165-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/97b89cc24f95/jfb-14-00165-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/d8feca409d34/jfb-14-00165-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/c45bd1fa000f/jfb-14-00165-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/052ec9de7d36/jfb-14-00165-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/7c96023c370e/jfb-14-00165-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5279/10055851/7c152ff3c9b1/jfb-14-00165-g006.jpg

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