Kamal Neel, Parshad Jagdish, Saharan Baljeet Singh, Kayasth Monika, Mudgal Vishal, Duhan Joginder Singh, Mandal Balwan Singh, Sadh Pardeep Kumar
Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, India.
Central Institute for Research on Buffaloes, Hisar 125001, India.
J Xenobiot. 2023 Mar 9;13(1):159-171. doi: 10.3390/jox13010013.
The current study emphasizes fungi as an important tool against heavy metals and how isolated fungal species can be used to create a successful strategy for the bioremediation of chromium and arsenic-contaminated sites/soils. Globally, heavy metal pollution is a serious issue. In the current investigation, contaminated sites were chosen, and samples could be taken from various localities of Hisar (29.1492° N, 75.7217° E) and Panipat (29.3909° N, 76.9635° E), India. A total of 19 fungal isolates were obtained from the collected samples through the enrichment culture technique using PDA media supplemented with Cr as chromic chloride hexahydrate (50 mg/L) and As as sodium arsenate (10 mg/L) and the potential of fungal isolates to be used for the removal of heavy metals was examined. The isolates were screened for minimum inhibitory concentrations (MIC) exhibiting tolerance capabilities, and the four best isolates C1, C3, A2, and A6 with the highest MICs (>5000 mg/L), were chosen for further investigations. To use the chosen isolates in the remediation of heavy metals (Cr and As), the culture conditions were optimized. The fungal isolates C1 and C3 estimated the highest removal of 58.60% and 57.00% at 50 mg/L chromium concentration, while the isolates A6 and A2 recorded the highest removal efficiency of 80% and 56% at 10 mg/L arsenic concentration under optimal conditions. Finally, the chosen fungal isolates C1 and A6 were molecularly identified as and , respectively.
当前的研究强调真菌作为对抗重金属的一种重要工具，以及如何利用分离出的真菌物种来制定一项成功的策略，用于对受铬和砷污染的场地/土壤进行生物修复。在全球范围内，重金属污染是一个严重的问题。在当前的调查中，选择了受污染的场地，并从印度希萨尔（北纬29.1492°，东经75.7217°）和潘尼帕特（北纬29.3909°，东经76.9635°）的不同地点采集样本。通过富集培养技术，使用添加了六水合氯化铬（50毫克/升）形式的铬和砷酸钠（10毫克/升）形式的砷的马铃薯葡萄糖琼脂（PDA）培养基，从采集的样本中总共获得了19株真菌分离物，并检测了这些真菌分离物用于去除重金属的潜力。对这些分离物进行最低抑菌浓度（MIC）筛选以显示其耐受能力，选择了MIC最高（>5000毫克/升）的四个最佳分离物C1、C3、A2和A6进行进一步研究。为了将所选分离物用于重金属（铬和砷）的修复，对培养条件进行了优化。在50毫克/升铬浓度下，真菌分离物C1和C3的去除率估计最高，分别为58.60%和57.00%，而在最佳条件下，分离物A6和A2在10毫克/升砷浓度下的去除效率最高，分别为80%和56%。最后，所选真菌分离物C1和A6经分子鉴定分别为 和 。
