18 种来自安康鱼肝的新型生物活性肽：生产、鉴定、抗氧化活性和稳定性。

Eighteen Novel Bioactive Peptides from Monkfish () Swim Bladders: Production, Identification, Antioxidant Activity, and Stability.

机构信息

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.

National and Provincial Joint Laboratory of Exploration, Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

出版信息

Mar Drugs. 2023 Mar 7;21(3):169. doi: 10.3390/md21030169.

In the study, papain was chosen from five proteases to hydrolyze proteins of monkfish swim bladders for effectively utilizing monkfish () processing byproducts, and the hydrolysis conditions of papain were optimized as hydrolysis temperature of 65 °C, pH 7.5, enzyme dose 2.5% and time 5 h using single-factor and orthogonal experiments. Eighteen peptides were purified from the swim bladder hydrolysate of monkfish by ultrafiltration and gel permeation chromatography methods and identified as YDYD, QDYD, AGPAS, GPGPHGPSGP, GPK, HRE, GRW, ARW, GPTE, DDGGK, IGPAS, AKPAT, YPAGP, DPT, FPGPT, GPGPT, GPT and DPAGP, respectively. Among eighteen peptides, GRW and ARW showed significant DPPH· scavenging activities with EC values of 1.053 ± 0.003 and 0.773 ± 0.003 mg/mL, respectively; YDYD, QDYD, GRW, ARW and YPAGP revealed significantly HO· scavenging activities with EC values of 0.150 ± 0.060, 0.177 ± 0.035, 0.201 ± 0.013, 0.183 ± 0.0016 and 0.190 ± 0.010 mg/mL, respectively; YDYD, QDYD, ARW, DDGGK and YPAGP have significantly O2-· scavenging capability with EC values of 0.126 ± 0.0005, 0.112 ± 0.0028, 0.127 ± 0.0002, 0.128 ± 0.0018 and 0.107 ± 0.0002 mg/mL, respectively; and YDYD, QDYD and YPAGP showed strong ABTS· scavenging ability with EC values of 3.197 ± 0.036, 2.337 ± 0.016 and 3.839 ± 0.102 mg/mL, respectively. YDYD, ARW and DDGGK displayed the remarkable ability of lipid peroxidation inhibition and Ferric-reducing antioxidant properties. Moreover, YDYD and ARW can protect Plasmid DNA and HepG2 cells against HO-induced oxidative stress. Furthermore, eighteen isolated peptides had high stability under temperatures ranging from 25-100 °C; YDYD, QDYD, GRW and ARW were more sensitive to alkali treatment, but DDGGK and YPAGP were more sensitive to acid treatment; and YDYD showed strong stability treated with simulated GI digestion. Therefore, the prepared antioxidant peptides, especially YDYD, QDYD, GRW, ARW, DDGGK and YPAGP from monkfish swim bladders could serve as functional components applied in health-promoting products because of their high-antioxidant functions.

在这项研究中，选择木瓜蛋白酶从五种蛋白酶中水解鳕鱼鳔的蛋白质，以有效利用鳕鱼（）加工副产物，并通过单因素和正交实验优化了木瓜蛋白酶的水解条件，即水解温度 65°C、pH7.5、酶剂量 2.5%和时间 5 小时。通过超滤和凝胶渗透色谱法从鳕鱼鳔水解物中纯化出 18 种肽，并分别鉴定为 YDYD、QDYD、AGPAS、GPGPHGPSGP、GPK、HRE、GRW、ARW、GPTE、DDGGK、IGPAS、AKPAT、YPAGP、DPT、FPGPT、GPGPT、GPT 和 DPAGP。在这 18 种肽中，GRW 和 ARW 表现出显著的 DPPH·清除活性，EC 值分别为 1.053±0.003 和 0.773±0.003mg/mL；YDYD、QDYD、GRW、ARW 和 YPAGP 表现出显著的 HO·清除活性，EC 值分别为 0.150±0.060、0.177±0.035、0.201±0.013、0.183±0.0016 和 0.190±0.010mg/mL；YDYD、QDYD、ARW、DDGGK 和 YPAGP 具有显著的 O2-·清除能力，EC 值分别为 0.126±0.0005、0.112±0.0028、0.127±0.0002、0.128±0.0018 和 0.107±0.0002mg/mL；YDYD、QDYD 和 YPAGP 表现出较强的 ABTS·清除能力，EC 值分别为 3.197±0.036、2.337±0.016 和 3.839±0.102mg/mL。YDYD、ARW 和 DDGGK 表现出较强的抑制脂质过氧化和铁还原抗氧化能力。此外，YDYD 和 ARW 可以保护质粒 DNA 和 HepG2 细胞免受 HO 诱导的氧化应激。此外，十八种分离肽在 25-100°C 的温度范围内具有较高的稳定性；YDYD、QDYD、GRW 和 ARW 对碱处理更敏感，但 DDGGK 和 YPAGP 对酸处理更敏感；YDYD 经模拟 GI 消化处理后稳定性较强。因此，从鳕鱼鳔中制备的抗氧化肽，特别是 YDYD、QDYD、GRW、ARW、DDGGK 和 YPAGP，由于其高抗氧化功能，可以作为功能成分应用于促进健康的产品中。