Morris J A, Oates K, Staff W G
Br J Urol. 1986 Apr;58(2):183-7. doi: 10.1111/j.1464-410x.1986.tb09023.x.
Four adenomatoid tumours of the epididymis were embedded in paraffin wax, sectioned and the sectioned surfaces examined by scanning electron microscopy using the secondary electron mode. The solid faces of the sectioned neoplasms were damaged by the preparative technique but the depressed luminal surfaces of the tubules were well preserved and yielded useful information. Large areas of luminal surface were examined and the presence and distribution of microvilli were noted. In addition, there was a more complex cell surface pattern of intersecting cord-like elevations. Desquamating cells were seen within the tubules and the cytoplasmic bridges noted by light microscopy were shown to be thin cytoplasmic partitions. These findings are consistent with the mesothelial theory of histogenesis. Scanning electron microscopy can provide useful information about the internal structure of neoplasms even with simple preparative techniques.
将四个附睾腺瘤样瘤嵌入石蜡中，切片，并使用二次电子模式通过扫描电子显微镜检查切片表面。切片肿瘤的实体面因制备技术而受损，但小管的凹陷腔面保存良好，并提供了有用的信息。检查了大面积的腔面，并记录了微绒毛的存在和分布。此外，还有一种更复杂的细胞表面模式，即相互交叉的索状隆起。在小管内可见脱落的细胞，光学显微镜下观察到的细胞质桥显示为薄的细胞质分隔。这些发现与组织发生的间皮理论一致。即使采用简单的制备技术，扫描电子显微镜也能提供有关肿瘤内部结构的有用信息。
