Enhancing the Toxicity of Cypermethrin and Spinosad against (Lepidoptera: Noctuidae) by Inhibition of Detoxification Enzymes.

The extensive use of wide-ranging insecticides in agricultural activities may develop resistance in insects. The dipping technique was utilized for examining changes in detoxifying enzyme levels in L. induced by cypermethrin (CYP) and spinosad (SPD) with and without a combination of three enzyme inhibitors: triphenyl phosphate (TPP), diethyl maleate (DEM), and piperonyl butoxide (PBO), at 70 μg/mL. PBO, DEM, and TPP showed 50% mortality against larvae at 236.2, 324.5, and 245.8 μg/mL, respectively. The LC value of CYP on larvae reduced from 2.86 μg/mL to 1.58, 2.26, and 1.96 μg/mL, while the LC value of SPD declined from 3.27 μg/mL to 2.34, 2.56, and 2.53, with the addition of PBO, DEM, and TPP, respectively, 24 h after treatment. Moreover, the activity of carboxylesterase (CarE), glutathione S-transferase (GST), and cytochrome P450 monooxygenase (Cyp 450) was significantly inhibited ( < 0.05) by TPP, DEM, PBO plus CYP, and SPD in larvae in comparison with tested insecticides alone. These findings suggested that three enzyme inhibitors play a major role in increasing the toxicity of CYP and SPD in and will provide insight into how to overcome insecticide resistance in insects.