Aioub Ahmed A A, Hashem Ahmed S, El-Sappah Ahmed H, El-Harairy Amged, Abdel-Hady Amira A A, Al-Shuraym Laila A, Sayed Samy, Huang Qiulan, Abdel-Wahab Sarah I Z
Plant Protection Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
Stored Product Pests Research Department, Plant Protection Research Institute, Agricultural Research Center, Sakha, Kafr El-Sheikh 33717, Egypt.
Toxics. 2023 Jun 19;11(6):542. doi: 10.3390/toxics11060542.
Insect glutathione S-transferases (GSTs) serve critical roles in insecticides and other forms of xenobiotic chemical detoxification. The fall armyworm, (J. E. Smith), is a major agricultural pest in several countries, especially Egypt. This is the first study to identify and characterize GST genes in under insecticidal stress. The present work evaluated the toxicity of emamectin benzoate (EBZ) and chlorantraniliprole (CHP) against the third-instar larvae of using the leaf disk method. The LC values of EBZ and CHP were 0.029 and 1.250 mg/L after 24 h of exposure. Moreover, we identified 31 GST genes, including 28 cytosolic and 3 microsomal SfGSTs from a transcriptome analysis and the genome data of . Depending on the phylogenetic analysis, sfGSTs were divided into six classes (delta, epsilon, omega, sigma, theta, and microsomal). Furthermore, we investigated the mRNA levels of 28 GST genes using qRT-PCR under EBZ and CHP stress in the third-instar larvae of . Interestingly, SfGSTe10 and SfGSTe13 stood out with the highest expression after the EBZ and CHP treatments. Finally, a molecular docking model was constructed between EBZ and CHP using the most upregulated genes (SfGSTe10 and SfGSTe13) and the least upregulated genes (SfGSTs1 and SfGSTe2) of larvae. The molecular docking study showed EBZ and CHP have a high binding affinity with SfGSTe10, with docking energy values of -24.41 and -26.72 kcal/mol, respectively, and sfGSTe13, with docking energy values of -26.85 and -26.78 kcal/mol, respectively. Our findings are important for understanding the role of GSTs in regarding detoxification processes for EBZ and CHP.
昆虫谷胱甘肽S-转移酶（GSTs）在杀虫剂及其他形式的外源化学物质解毒过程中发挥着关键作用。草地贪夜蛾，（J. E. 史密斯），是多个国家尤其是埃及的主要农业害虫。这是第一项在杀虫胁迫下鉴定和表征草地贪夜蛾GST基因的研究。本研究采用叶碟法评估了甲氨基阿维菌素苯甲酸盐（EBZ）和氯虫苯甲酰胺（CHP）对草地贪夜蛾三龄幼虫的毒性。暴露24小时后，EBZ和CHP的LC值分别为0.029和1.250 mg/L。此外，我们通过转录组分析和草地贪夜蛾的基因组数据鉴定出31个GST基因，包括28个胞质型和3个微粒体型SfGSTs。根据系统发育分析，sfGSTs被分为六类（δ、ε、ω、σ、θ和微粒体型）。此外，我们在草地贪夜蛾三龄幼虫的EBZ和CHP胁迫下，使用qRT-PCR研究了28个GST基因的mRNA水平。有趣的是，SfGSTe10和SfGSTe13在EBZ和CHP处理后表现出最高的表达水平。最后，利用草地贪夜蛾幼虫上调最多的基因（SfGSTe10和SfGSTe13）和上调最少的基因（SfGSTs1和SfGSTe2）构建了EBZ和CHP之间的分子对接模型。分子对接研究表明，EBZ和CHP与SfGSTe10具有高结合亲和力，对接能值分别为-24.41和-26.72 kcal/mol，与sfGSTe13的对接能值分别为-26.85和-26.78 kcal/mol。我们的研究结果对于理解GSTs在草地贪夜蛾对EBZ和CHP解毒过程中的作用具有重要意义。
