约旦儿童抗菌药物耐药性的分子机制：一项横断面观察性研究。
Antimicrobial Resistance Molecular Mechanisms of in Jordanian Children: A Cross-Sectional Observational Study.
作者信息
Burayzat Salma, Al-Tamimi Mohammad, Barqawi Mohammad, Massadi Mustafa Sabri, Abu-Raideh Jumanah, Albalawi Hadeel, Khasawneh Ashraf I, Himsawi Nisreen, Barber Maha
机构信息
Department of Pediatrics and Neonatology, Faculty of Medicine, The Hashemite University, Zarqa 13133, Jordan.
Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, Zarqa 13133, Jordan.
出版信息
Antibiotics (Basel). 2023 Mar 20;12(3):618. doi: 10.3390/antibiotics12030618.
BACKGROUND
antimicrobial resistance causes increasing treatment failure rates among gastritis in children. This study investigates the molecular mechanisms of antimicrobial resistance among Jordanian children.
METHODS
Demographic, clinical, and laboratory data were recorded for children referred to Prince Hamzah Hospital. Clarithromycin, Metronidazole, and Levofloxacin susceptibility were tested via E-test. Clarithromycin-related mutations were investigated using Real-Time (RT)-PCR and Levofloxacin resistance was analyzed with DNA sequencing of the gene.
RESULTS
116 children were recruited, including 55.2% females and 55.2% in the age range of 10.1 to 14 years. A total of 82.7% were naïve to eradication therapy. positivity was 93.9%, 89.6%, 61.7%, and 84.3% according to Rapid Urease Test, histology, culture, and RT-PCR, respectively. Resistance rates were 25.9% for Clarithromycin, 50% for Metronidazole, and 6.9% for Levofloxacin via E-test. A2142G or A2143G or a combination of both mutations concerning Clarithromycin resistance were documented in 26.1% of samples, while mutations in gen-related to Levofloxacin resistance were reported in 5.3% of samples. Antibiotic resistance was significantly affected by abdominal pain, anemia, hematemesis, and histological findings ( < 0.05).
CONCLUSION
resistance was documented for Metronidazole and Clarithromycin. RT-PCR for identification and microbial resistance determination are valuable alternatives for cultures in determining antimicrobial susceptibility.
背景
抗生素耐药性导致儿童胃炎的治疗失败率不断上升。本研究调查了约旦儿童抗生素耐药性的分子机制。
方法
记录转诊至哈姆扎王子医院儿童的人口统计学、临床和实验室数据。通过E-test检测克拉霉素、甲硝唑和左氧氟沙星的敏感性。使用实时（RT）-PCR研究克拉霉素相关突变，并通过该基因的DNA测序分析左氧氟沙星耐药性。
结果
共招募了116名儿童，其中55.2%为女性，55.2%年龄在10.1至14岁之间。共有82.7%的儿童未接受过根除治疗。根据快速尿素酶试验、组织学、培养和RT-PCR，阳性率分别为93.9%、89.6%、61.7%和84.3%。通过E-test检测，克拉霉素耐药率为25.9%，甲硝唑为50%，左氧氟沙星为6.9%。26.1%的样本记录了与克拉霉素耐药相关的A2142G或A2143G或两者组合的突变，而5.3%的样本报告了与左氧氟沙星耐药相关的基因中的突变。腹痛、贫血、呕血和组织学结果对抗生素耐药性有显著影响（P<0.05）。
结论
记录到甲硝唑和克拉霉素耐药。用于鉴定和微生物耐药性测定的RT-PCR是确定抗菌药物敏感性时替代培养的有价值方法。
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