罗马尼亚东北部儿科患者对克拉霉素的原发性耐药模式
Pattern of Primary Resistance of to Clarithromycin among Pediatric Patients from North-Eastern Romania.
作者信息
Rosu Oana-Maria, Gimiga Nicoleta, Popescu Roxana, Ioniuc Ileana, Rusu Carmen Daniela, Clipa Tatiana, Florea Diana-Maria, Pleșca Doina-Anca, Nemtoi Alexandru, Tataranu Elena, Stefanescu Gabriela, Diaconescu Smaranda
机构信息
Doctoral School, "George Emil Palade" University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Str., 540139 Targu Mures, Romania.
Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania.
出版信息
Children (Basel). 2023 Oct 28;10(11):1752. doi: 10.3390/children10111752.
BACKGROUND
antibiotic resistance has increased worldwide and affects the effectiveness of current therapies. The recommended first-line empiric treatment should be tailored to the local clarithromycin resistance rate. This study aimed to determine the pediatric patient profile and rate of clarithromycin resistance for patients diagnosed with by gastric biopsy.
METHODS
We studied 84 positive gastric samples for . Positive results were confirmed by a rapid urease test and histopathological examination, with the type of gastritis established according to the Sydney System. Gastric biopsy samples were stored in RNA saver. Clarithromycin resistance was determined by a real-time polymerase chain reaction-based molecular assay after RNA-DNA extraction.
RESULTS
Of the 84 biopsy samples analyzed, 35 (41.6%) were resistant to clarithromycin. Clarithromycin resistance was found mainly in girls (80%) with a mean age of 15 years (range 6-17 years). The history of prior exposure to clarithromycin was 91.6%. The concordance between the histopathological examination and the PCR test was 100%.
CONCLUSIONS
One in 2.4 children infected with had a strain resistant to clarithromycin. This resistant strain may be a reason for treatment failure in Romanian children, yet this is uninvestigated. The high rate of bacterial resistance to this antibiotic among children indicates the need for susceptibility testing before therapy.
背景
抗生素耐药性在全球范围内有所增加，影响了当前治疗方法的有效性。推荐的一线经验性治疗应根据当地克拉霉素耐药率进行调整。本研究旨在确定经胃活检诊断为[具体疾病名称未给出]的儿科患者特征及克拉霉素耐药率。
方法
我们研究了84份[具体疾病名称未给出]的胃阳性样本。通过快速尿素酶试验和组织病理学检查确认阳性结果，并根据悉尼系统确定胃炎类型。胃活检样本保存在RNA保存液中。在进行RNA-DNA提取后，通过基于实时聚合酶链反应的分子检测法测定克拉霉素耐药性。
结果
在分析的84份活检样本中，35份（41.6%）对克拉霉素耐药。克拉霉素耐药主要见于女孩（80%），平均年龄15岁（范围6 - 17岁）。既往接触克拉霉素的病史为91.6%。组织病理学检查与PCR检测的一致性为100%。
结论
每2.4名感染[具体疾病名称未给出]的儿童中就有1名携带对克拉霉素耐药的菌株。这种耐药菌株可能是罗马尼亚儿童治疗失败的原因，但尚未对此进行调查。儿童中该抗生素的高耐药率表明在治疗前需要进行药敏试验。
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