Loh Kar-Hoe, Lim Kean-Chong, Then Amy Yee-Hui, Adam Serena, Leung Amanda Jhu-Xhin, Hu Wenjia, Bong Chui Wei, Wang Aijun, Sade Ahemad, Musel Jamil, Du Jianguo
Institute of Ocean and Earth Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Animals (Basel). 2023 Mar 9;13(6):1002. doi: 10.3390/ani13061002.
The data provided in this article are partial fragments of the Cytochrome c oxidase subunit 1 mitochondrial gene (CO1) sequences of 175 tissues sampled from sharks and batoids collected from Malaysian waters, from June 2015 to June 2022. The barcoding was done randomly for six specimens from each species, so as to authenticate the code. We generated barcodes for 67 different species in 20 families and 11 orders. DNA was extracted from the tissue samples following the Chelex protocols and amplified by polymerase chain reaction (PCR) using the barcoding universal primers FishF2 and FishR2. A total of 654 base pairs (bp) of barcode CO1 gene from 175 samples were sequenced and analysed. The genetic sequences were blasted into the NCBI GenBank and Barcode of Life Data System (BOLD). A review of the blast search confirmed that there were 68 valid species of sharks and batoids that occurred in Malaysian waters. We provided the data of the COI gene mid-point rooting phylogenetic relation trees and analysed the genetic distances among infra-class and order, intra-species, inter-specific, inter-genus, inter-familiar, and inter-order. We confirmed the addition of , , and as new records for Malaysia. The establishment of a comprehensive CO1 database for sharks and batoids will help facilitate the rapid monitoring and assessment of elasmobranch fisheries using environmental DNA methods.
本文提供的数据是2015年6月至2022年6月从马来西亚海域采集的鲨鱼和鳐类动物的175个组织样本的细胞色素c氧化酶亚基1线粒体基因（CO1）序列的部分片段。对每个物种的六个标本随机进行条形码编码，以验证编码。我们为11目20科的67个不同物种生成了条形码。按照Chelex方案从组织样本中提取DNA，并使用条形码通用引物FishF2和FishR2通过聚合酶链反应（PCR）进行扩增。对175个样本的条形码CO1基因的总共654个碱基对（bp）进行了测序和分析。将遗传序列与NCBI基因库和生命条形码数据系统（BOLD）进行比对。比对搜索结果显示，马来西亚海域有68种有效的鲨鱼和鳐类物种。我们提供了COI基因中点生根系统发育关系树的数据，并分析了亚纲和目、种内、种间、属间、科间和目间的遗传距离。我们确认新增了 、 、 和 作为马来西亚的新记录。建立一个全面的鲨鱼和鳐类CO1数据库将有助于利用环境DNA方法快速监测和评估板鳃亚纲渔业。
