Lubkowska Anna, Dudzińska Wioleta, Pluta Waldemar
Department of Functional Diagnostics and Physical Medicine, Pomeranian Medical University in Szczecin, Żołnierska 54, 71-210 Szczecin, Poland.
Department of Physiology and Biochemistry, Institute of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland.
Antioxidants (Basel). 2023 Mar 6;12(3):655. doi: 10.3390/antiox12030655.
Oxidants are generated by all cells during normal oxidative respiration, and as long as they are under the control of appropriate mechanisms, they act as intracellular signaling molecules participating in complex functions. Oxidative stress can also affect insulin levels in the body. The production of reactive oxygen species by-products can lead to insulin resistance. Heat shock proteins (70 kDa) protect cells from the damaging effects of heat shock but also oxidative stress. The aim of the study was to investigate the serum concentration of HSP70 in young, non-obese but overweight men (BMI ≤ 30 kg/m) and to assess its association with the insulin resistance, lipid profile and antioxidant system of red blood cells. Fifty-seven young men were examined and divided into two groups: lean men (n = 30) and men overweight (n = 27). A statistically significant difference was observed in the BMI ( < 0.007), HSP70 concentration ( < 0.000), serum insulin concentration ( < 0.000), HOMA-IR ( < 0.0001), superoxide dismutase ( < 0.02) and glutathione peroxidase ( < 0.05) between the studied groups. There was a negative correlation between the concentration of HSP70 with the insulin level (r = -0.50; < 0.0004) and with the HOMA-IR (r = -0.50; < 0.0004). These changes were associated with an increase in the activity of antioxidant enzymes. Our findings suggest that measuring the extracellular concentration of HSP70 can be an important indicator in disorders of glucose homeostasis.
在正常氧化呼吸过程中，所有细胞都会产生氧化剂，只要它们处于适当机制的控制之下，就会作为细胞内信号分子参与复杂的功能。氧化应激也会影响体内胰岛素水平。活性氧副产物的产生会导致胰岛素抵抗。热休克蛋白（70 kDa）可保护细胞免受热休克的破坏作用，也能抵御氧化应激。本研究的目的是调查年轻、非肥胖但超重男性（BMI≤30 kg/m）血清中HSP70的浓度，并评估其与胰岛素抵抗、血脂谱和红细胞抗氧化系统的关系。对57名年轻男性进行了检查，并将其分为两组：瘦男性（n = 30）和超重男性（n = 27）。研究组之间在BMI（<0.007）、HSP70浓度（<0.000）、血清胰岛素浓度（<0.000）、HOMA-IR（<0.0001）、超氧化物歧化酶（<0.02）和谷胱甘肽过氧化物酶（<0.05）方面存在统计学上的显著差异。HSP70浓度与胰岛素水平（r = -0.50；<0.0004）和HOMA-IR（r = -0.50；<0.0004）之间呈负相关。这些变化与抗氧化酶活性的增加有关。我们的研究结果表明，测量HSP70的细胞外浓度可能是葡萄糖稳态紊乱的一个重要指标。
