Han Mei, Yang Fengxian, Zhang Kun, Ni Jiyan, Zhao Xia, Chen Xuelin, Zhang Zhennan, Wang Hanlei, Lu Jing, Zhang Yumei
Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China.
Faculty of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
Antioxidants (Basel). 2023 Mar 8;12(3):664. doi: 10.3390/antiox12030664.
is a traditional Dai medicine plant belonging to the Lamiaceae family, which can be used to treat malaria, inflammation, diabetes, liver disease, bronchitis, tumors, cholelithiasis, jaundice, skin disease and as an anti-helminthic. To find more novel therapeutic agents contained in this medicinal plant, the antioxidant, anti-inflammatory and anti-diabetic activities of methanolic extract, fractions and compounds were evaluated. In this study, 26 compounds were isolated from the leaves and branches of . Their structures were identified based on extensive spectral experiments, including NMR, ESI-MS and comparison with published spectral data. Among them, compounds -, -, - and - were reported for the first time for this plant. The antioxidant activity screening results showed that compounds , and had potent antioxidant capacities, with SC values from 0.32 to 9.92 µmol/L, 0.92 to 1.10 mmol Trolox/L and 1.02 to 1.22 mmol Trolox/L for DPPH, ABTS and FRAP, respectively. In addition, their anti-inflammatory effects were investigated by releasing TNF-, IL-1 and IL-6 through the use of mouse monocytic macrophages (RAW 264.7). Compounds , , and had the effects of reducing the expression of inflammatory factors. Compounds and were reported for the first time for their anti-inflammatory activities. Furthermore, the methanolic extract (ME), petroleum ether extract (PEE) and EtOAc extract (EAE) of showed significant glucose uptake activities; compounds and significantly promoted glucose uptake of 3T3-L1 adipocytes at 40 µM. Meanwhile, compounds , and showed significant inhibitory activities against α-glucosidase, with IC values of 14.16 ± 0.34 µmol/L, 19.29 ± 0.26 µmol/L and 3.04 ± 0.08 µmol/L, respectively. Compounds and were reported for the first time for their -glucosidase inhibitory activities. Our investigation explored the possible therapeutic material basis of to prevent oxidative stress and related diseases, especially inflammation and diabetes.
是一种属于唇形科的传统傣药植物，可用于治疗疟疾、炎症、糖尿病、肝病、支气管炎、肿瘤、胆结石、黄疸、皮肤病以及作为抗蠕虫药。为了找到这种药用植物中含有的更多新型治疗剂，对甲醇提取物、馏分和化合物的抗氧化、抗炎和抗糖尿病活性进行了评估。在本研究中，从的叶和枝中分离出26种化合物。基于广泛的光谱实验，包括核磁共振（NMR）、电喷雾电离质谱（ESI-MS）并与已发表的光谱数据进行比较，确定了它们的结构。其中，化合物-、-、-和-首次在此植物中被报道。抗氧化活性筛选结果表明，化合物、和具有较强的抗氧化能力，对二苯基苦味酰基自由基（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和铁离子还原抗氧化能力（FRAP）的半抑制浓度（SC）值分别为0.32至9.92 μmol/L、0.92至1.10 mmol Trolox/L和1.02至1.22 mmol Trolox/L。此外，通过使用小鼠单核巨噬细胞（RAW 264.7）释放肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）来研究它们的抗炎作用。化合物、、和具有降低炎症因子表达的作用。化合物和的抗炎活性首次被报道。此外，的甲醇提取物（ME）、石油醚提取物（PEE）和乙酸乙酯提取物（EAE）表现出显著的葡萄糖摄取活性；化合物和在40 μM时显著促进3T3-L1脂肪细胞的葡萄糖摄取。同时，化合物、和对α-葡萄糖苷酶表现出显著的抑制活性，半数抑制浓度（IC）值分别为14.16±0.34 μmol/L、19.29±0.26 μmol/L和3.04±0.08 μmol/L。化合物和的α-葡萄糖苷酶抑制活性首次被报道。我们的研究探索了预防氧化应激及相关疾病，尤其是炎症和糖尿病的可能治疗物质基础。
