Phytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa.
Department of Chemistry, Makerere University, Kampala P.O. Box 7062, Uganda.
Molecules. 2022 Dec 15;27(24):8912. doi: 10.3390/molecules27248912.
The present study investigated phenolic compounds, antioxidant, antidiabetic, and the anti-inflammatory potentials of methanolic and chloroform extracts of . The methanolic extract included, polyphenols (112 ± 2.81 mg gallic acid equivalent (GAE)/g), flavonols (76.12 ± 7.95 mg quercetin equivalents (QE)/g); antioxidant capacity (Ferric Reducing Antioxidant Power (FRAP) (752.64 ± 89.0 μmol of ascorbic acid equivalents (AAE) per g dry weight (µmol AAE/g), 2,2-dyphenyl-1-picrylhydrazyl (DPPH) (812.18 ± 51.12 Trolox equivalents per gram of dry mass of plant extracts (μmol TE/g), TEAC (631.63 ± 17.42 µmol TE/g)), while the chloroform extract included polyphenols (39.93 ± 1.36 mg GAE/g), flavonols (44.81 ± 3.74 mg QE/g); antioxidant capacity, DPPH (58.70 ± 5.18 µmol TE/g), TEAC (118.63 ± 3.74 µmol TE/g) and FRAP (107.10 ± 2.41 µmol AAE/g). The phytochemicals profiling performed by UPLC-ESI-QTOF-MS revealed some important polyphenols, predominantly flavonoids, that could be responsible for the antioxidant capacity and biological effects. Both extracts demonstrated a dose-dependent manner of the alpha-glucosidase inhibition with an IC between 125 and 250 μg/mL for methanolic extract, while the chloroform extract was at 250 μg/mL. In the L6 myoblasts and C3A hepatocytes, the methanolic extract slightly increased the utilization of glucose, and both extracts exhibited a dose-dependent increase in the glucose uptake in both cell types without significantly increasing the cytotoxicity. Furthermore, both extracts exhibited an anti-inflammatory potential and the findings from the present study could serve as a baseline for further research in the development of pharmaceutical agents.
本研究调查了 甲醇和氯仿提取物的酚类化合物、抗氧化、抗糖尿病和抗炎潜力。甲醇提取物包括多酚（112±2.81mg 没食子酸当量（GAE）/g）、类黄酮（76.12±7.95mg 槲皮素当量（QE）/g）；抗氧化能力（铁还原抗氧化能力（FRAP）（752.64±89.0μmol 抗坏血酸当量（AAE）/g 干重（µmol AAE/g）、2,2-二苯基-1-苦基肼基（DPPH）（812.18±51.12 植物提取物每克干重的生育酚当量（μmol TE/g）、TEAC（631.63±17.42μmol TE/g）），而氯仿提取物包括多酚（39.93±1.36mg GAE/g）、类黄酮（44.81±3.74mg QE/g）；抗氧化能力、DPPH（58.70±5.18μmol TE/g）、TEAC（118.63±3.74μmol TE/g）和 FRAP（107.10±2.41μmol AAE/g）。UPLC-ESI-QTOF-MS 进行的植物化学成分分析揭示了一些重要的多酚，主要是类黄酮，它们可能是抗氧化能力和生物效应的原因。两种提取物均表现出对 α-葡萄糖苷酶抑制的剂量依赖性，甲醇提取物的 IC 介于 125 和 250μg/mL 之间，而氯仿提取物则为 250μg/mL。在 L6 成肌细胞和 C3A 肝细胞中，甲醇提取物轻微增加葡萄糖的利用，两种提取物均表现出剂量依赖性增加两种细胞类型的葡萄糖摄取，而细胞毒性无明显增加。此外，两种提取物均表现出抗炎潜力，本研究的结果可以为进一步研究开发药物制剂提供基础。
