通过溶菌酶负载和可见光照射定制的ZnSe、ZnSe-TiO和TiO颗粒的抗菌活性

Antibacterial Activity of ZnSe, ZnSe-TiO and TiO Particles Tailored by Lysozyme Loading and Visible Light Irradiation.

作者信息

Anastasescu Crina, Neagu Simona, Preda Silviu, Culita Daniela, Stancu Mihaela, Banciu Cristian, Munteanu Cornel, Bratan Veronica, Calderon-Moreno Jose Maria, State Razvan, Anastasescu Mihai, Enache Madalin, Balint Ioan, Zaharescu Maria

机构信息

"Ilie Murgulescu" Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania.

Institute of Biology of Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, Romania.

出版信息

Antioxidants (Basel). 2023 Mar 10;12(3):691. doi: 10.3390/antiox12030691.

DOI:10.3390/antiox12030691

PMID:36978939

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10045246/

Abstract

ZnSe, ZnSe-TiO microspheres and nanostructured TiO obtained by hydrothermal and sol-gel methods were tested against ATCC 25923 and ATCC 4698 before and after lysozyme (Lys) loading. Morphological characterization of inorganic matrices and hybrid organic-inorganic complexes were performed by microscopy techniques (SEM, AFM and Dark Field Hyperspectral Microscopy). Light absorption properties of ZnSe, ZnSe-TiO and TiO powders were assessed by UV-visible spectroscopy and their ability to generate reactive oxygen species (•OH and O) under visible light irradiation was investigated. Antibacterial activity of ZnSe, ZnSe-TiO, TiO, Lys/ZnSe, Lys/ZnSe-TiO and Lys/TiO samples under exposure to visible light irradiation (λ > 420 nm) was tested against and and correlated with ROS photogeneration.

摘要

通过水热法和溶胶-凝胶法制备的ZnSe、ZnSe-TiO微球以及纳米结构TiO，在负载溶菌酶（Lys）前后，针对ATCC 25923和ATCC 4698进行了测试。通过显微镜技术（扫描电子显微镜、原子力显微镜和暗场高光谱显微镜）对无机基质和有机-无机杂化复合物进行了形态表征。通过紫外-可见光谱评估了ZnSe、ZnSe-TiO和TiO粉末的光吸收特性，并研究了它们在可见光照射下产生活性氧（•OH和O）的能力。测试了ZnSe、ZnSe-TiO、TiO、Lys/ZnSe、Lys/ZnSe-TiO和Lys/TiO样品在可见光照射（λ > 420 nm）下对ATCC 25923和ATCC 4698的抗菌活性，并将其与活性氧的光生成相关联。