Cui Yuxing, Wang Jingxuan, Bai Yingxue, Ban Liping, Ren Junda, Shang Qiaoxia, Li Weiyu
College of Plant Science and Technology, Beijing University of Agriculture/Beijing Key Laboratory of New Agricultural Technology in Agriculture Application/National Demonstration Center for Experimental Plant Production Education, Beijing 102206, China.
Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing 102206, China.
Biology (Basel). 2023 Mar 12;12(3):439. doi: 10.3390/biology12030439.
Cyclic nucleotide-gated channels (), non-selective cation channels localised on the plasmalemma, are involved in growth, development, and regulatory mechanisms in plants during adverse stress. To date, gene families in multiple crops have been identified and analysed. However, there have been no systematic studies on the evolution and development of gene families in legumes. Therefore, in the present study, via transcriptome analysis, we identified 143 genes in legumes, and thereafter, classified and named them according to the grouping method used for . Functional verification for disease stress showed that four were specifically expressed in the plasmalemma during the stress process. Further, functional enrichment analysis showed that their mode of participation and coordination included inorganic ion concentration regulation inside and outside the membrane via the transmembrane ion channel and participation in stress regulation via signal transduction. The family genes in involved in disease stress were also identified and physiological stress response and omics analyses were also performed. Our preliminary results revealed the basic laws governing the involvement of in disease resistance in , providing important gene resources and a theoretical reference for the breeding of resistant soybean.
环核苷酸门控通道（）是位于质膜上的非选择性阳离子通道，参与植物在逆境胁迫下的生长、发育和调控机制。迄今为止，已对多种作物中的基因家族进行了鉴定和分析。然而，尚未对豆科植物中基因家族的进化和发展进行系统研究。因此，在本研究中，通过转录组分析，我们在豆科植物中鉴定出143个基因，然后根据用于的分组方法对其进行分类和命名。病害胁迫的功能验证表明，有4个基因在胁迫过程中在质膜中特异性表达。此外，功能富集分析表明，它们的参与和协调模式包括通过跨膜离子通道调节膜内外的无机离子浓度以及通过信号转导参与胁迫调节。还鉴定了参与病害胁迫的中的家族基因，并进行了生理胁迫响应和组学分析。我们的初步结果揭示了参与抗病性的基本规律，为抗性大豆育种提供了重要的基因资源和理论参考。
