Laboratorio PABIOM, Facultad de Medicina y Ciencias Biomédicas, Universidad Autónoma de Chihuahua, Chihuahua 31125, Mexico.
Biomolecules. 2023 Mar 7;13(3):488. doi: 10.3390/biom13030488.
Lifestyle modifications in preclinical Rheumatoid Arthritis (RA) could delay the ongoing pathogenic immune processes and potentially prevent its onset. Physical exercise (PE) benefits RA patients; however, its impact in reducing the risk of developing RA has scarcely been studied. The objective was to describe the effects of low-intensity PE applied at the disease's preclinical phase on the joints of DBA/1 mice with collagen-induced arthritis (CIA). Twelve mice with CIA were randomly distributed into two groups: the CIA-Ex group, which undertook treadmill PE, and the CIA-NoEx, which was not exercised. The effects of PE were evaluated through clinical, histological, transcriptomics, and immunodetection analyses in the mice's hind paws. The CIA-Ex group showed lower joint inflammation and damage and a decreased expression of RA-related genes (, , , , and ) and signaling pathways (Cytokines, Chemokines, JAK-STAT, MAPK, NF-kappa B, TNF, and TGF-beta). TNF-α expression was decreased by PE in the inflamed joints. Low-intensity PE in pre-arthritic CIA reduced the severity through joint down-expression of proinflammatory genes and proteins. Knowledge on the underlying mechanisms of PE in preclinical arthritis and its impact on reducing the risk of developing RA is still needed.
在类风湿关节炎（RA）的临床前阶段进行生活方式的改变可能会延迟正在进行的致病免疫过程，并有可能预防其发病。体育锻炼（PE）有益于 RA 患者；然而，其在降低患 RA 的风险方面的影响却鲜有研究。本研究的目的是描述在胶原诱导性关节炎（CIA）的临床前阶段应用低强度 PE 对 DBA/1 小鼠关节的影响。12 只 CIA 小鼠被随机分为两组：进行跑步机 PE 的 CIA-Ex 组和不运动的 CIA-NoEx 组。通过对小鼠后爪进行临床、组织学、转录组学和免疫检测分析来评估 PE 的效果。CIA-Ex 组显示出较低的关节炎症和损伤，以及与 RA 相关的基因（、、、、和）和信号通路（细胞因子、趋化因子、JAK-STAT、MAPK、NF-kappa B、TNF 和 TGF-beta）的表达降低。PE 降低了炎症关节中 TNF-α的表达。在关节炎前期的 CIA 中进行低强度的 PE 通过下调促炎基因和蛋白减轻了关节的严重程度。关于 PE 在临床前关节炎中的潜在机制及其对降低患 RA 风险的影响的知识仍然需要进一步研究。
