Department of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USA.
Biomolecules. 2023 Mar 18;13(3):559. doi: 10.3390/biom13030559.
Infant colic is a common condition with unclear biologic underpinnings and limited treatment options. We hypothesized that complex molecular networks within human milk (i.e., microbes, micro-ribonucleic acids (miRNAs), cytokines) would contribute to colic risk, while controlling for medical, social, and nutritional variables. This hypothesis was tested in a cohort of 182 breastfed infants, assessed with a modified Infant Colic Scale at 1 month. RNA sequencing was used to interrogate microbial and miRNA features. Luminex assays were used to measure growth factors and cytokines. Milk from mothers of infants with colic ( = 28) displayed higher levels of (adj. = 0.038, = 0.30), miR-224-3p (adj. = 0.023, = 0.33), miR-125b-5p (adj. = 0.028, = 0.29), let-7a-5p (adj. = 0.028, = 0.27), and miR-205-5p (adj. = 0.029, = 0.26) compared to milk from non-colic mother-infant dyads ( = 154). Colic symptom severity was directly associated with milk hepatocyte growth factor levels ( = 0.21, = 0.025). A regression model involving let-7a-5p, miR-29a-3p, and accurately modeled colic risk ( = 16.7, = 0.001). Molecular factors within human milk may impact colic risk, and provide support for a dysbiotic/inflammatory model of colic pathophysiology.
婴儿肠绞痛是一种常见病症，其生物学基础尚不清楚，治疗选择有限。我们假设人乳中的复杂分子网络（即微生物、微小 RNA（miRNA）、细胞因子）将有助于确定肠绞痛风险，同时控制医疗、社会和营养变量。在 182 名母乳喂养的婴儿队列中测试了这一假设，这些婴儿在 1 个月时使用改良婴儿绞痛量表进行评估。使用 RNA 测序来检测微生物和 miRNA 特征。使用 Luminex 测定法测量生长因子和细胞因子。患有绞痛的婴儿（ = 28）的母亲的牛奶显示出更高水平的（adj. = 0.038， = 0.30），miR-224-3p（adj. = 0.023， = 0.33），miR-125b-5p（adj. = 0.028， = 0.29），let-7a-5p（adj. = 0.028， = 0.27）和 miR-205-5p（adj. = 0.029， = 0.26）与非绞痛母婴对（ = 154）的牛奶相比。绞痛症状严重程度与牛奶肝细胞生长因子水平直接相关（ = 0.21， = 0.025）。一个涉及 let-7a-5p、miR-29a-3p 和的回归模型准确地模拟了绞痛风险（ = 16.7， = 0.001）。人乳中的分子因素可能会影响绞痛风险，并为人绞痛病理生理学的失调/炎症模型提供支持。
