Chemistry Department, Yasouj University, Yasouj 75914-353, Iran.
Department of Chemistry, Farhangian University, Tehran 19396-14464, Iran.
Biosensors (Basel). 2023 Mar 12;13(3):374. doi: 10.3390/bios13030374.
Inspired by laccase activity, herein, Cu-guanosine nanorods (Cu-Guo NRs) have been synthesized for the first time through a simple procedure. The activity of the Cu-Guo NR as the laccase mimicking nanozyme has been examined in the colorimetric sensing of rutin (Rtn) by a novel and simple spectrophotometric method. The distinct changes in the absorbance signal intensity of Rtn and a distinguished red shift under the optimum condition based on pH and ionic strength values confirmed the formation of the oxidized form of Rtn (o-quinone) via laccase-like nanozyme activity of Cu-Guo NRs. A vivid and concentration-dependent color variation from green to dark yellow led to the visual detection of Rtn in a broad concentration range from 770 nM to 54.46 µM with a limit of detection (LOD) of 114 nM. The proposed methodology was successfully applied for the fast tracing of Rtn in the presence of certain common interfering species and various complex samples such as propolis dry extract, human biofluids, and dietary supplement tablets, with satisfactory precision. The sensitivity and selectivity of the developed sensor, which are bonuses in addition to rapid, on-site, cost-effective, and naked-eye determination of Rtn, hold great promise to provide technical support for routine analysis in the real world.
受漆酶活性的启发，本文首次通过简单的方法合成了铜-鸟嘌呤纳米棒（Cu-Guo NRs）。通过一种新颖而简单的分光光度法，研究了 Cu-Guo NR 作为漆酶模拟纳米酶在芦丁（Rtn）比色传感中的活性。在基于 pH 值和离子强度值的最佳条件下，Rtn 的吸光度信号强度的明显变化和明显的红移证实了通过 Cu-Guo NRs 的漆酶样纳米酶活性形成了 Rtn 的氧化形式（邻醌）。生动且与浓度相关的颜色变化从绿色变为深黄色，导致 Rtn 在从 770 nM 到 54.46 µM 的宽浓度范围内的可视化检测，检测限（LOD）为 114 nM。该方法已成功用于在存在某些常见干扰物质和各种复杂样品（如蜂胶干提取物、人体生物体液和膳食补充片剂）的情况下快速追踪 Rtn，具有令人满意的精密度。该传感器的灵敏度和选择性是快速、现场、经济高效和肉眼测定 Rtn 的额外优势，为实际分析中的常规分析提供了技术支持。
