Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj, Iran.
Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj, Iran; Research Center for Nanotechnology, University of Kurdistan, 66177-15175, Sanandaj, Iran.
Colloids Surf B Biointerfaces. 2020 Nov;195:111228. doi: 10.1016/j.colsurfb.2020.111228. Epub 2020 Jun 30.
A sensitive colorimetric and electrochemical sensor for measuring of epinephrine (EP) was developed based on CuO nanorods (NRs), and applicability of the sensor for detection of release epinephrine (EP) from living cells was evaluated. The CuO NRs was prepared using a facile and efficient method in low temperature and characterized by Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Energy-dispersive X-ray spectroscopy (EDX). The CuO NRs exhibited laccase-like activity and could oxidize epinephrine (EP) to a colored product. No interference from the common interfering agents such as dopamine, ascorbic acid and uric acid was observed. Colorimetric sensor demonstrated a linear range of 0.6-18 μM with detection limit of 0.31 μM. Furthermore, the electrochemical study showed CuO NRs exhibited excellent electrocatalytic activity towards epinephrine oxidation. Differential pulse voltammetry signals increase with increasing of EP concentration in the range 0.04-14 μM, with a detection limit of 20 nM. Finally, the proposed sensor applied to perform real-time monitoring of epinephrine released by PC12 cells, indicating that CuO NRs provide a new platform for developing high-performance sensors in biological applications.
基于 CuO 纳米棒（NRs）开发了一种用于测量肾上腺素（EP）的灵敏比色和电化学传感器，并评估了该传感器用于检测活细胞中释放的 EP 的适用性。通过在低温下使用简便有效的方法制备了 CuO NRs，并通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X 射线粉末衍射（XRD）、X 射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）和能量色散 X 射线光谱（EDX）对其进行了表征。CuO NRs 表现出漆酶样活性，可以将肾上腺素（EP）氧化为有色产物。没有观察到多巴胺、抗坏血酸和尿酸等常见干扰物的干扰。比色传感器显示出 0.6-18 μM 的线性范围，检测限为 0.31 μM。此外，电化学研究表明，CuO NRs 对肾上腺素氧化具有优异的电催化活性。在 0.04-14 μM 的范围内，随着 EP 浓度的增加，差分脉冲伏安法信号增加，检测限为 20 nM。最后，该传感器用于实时监测 PC12 细胞释放的肾上腺素，表明 CuO NRs 为开发生物应用中的高性能传感器提供了新平台。
