Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, China.
Department of Neurosurgery, First Hospital of Jilin University, Changchun, China.
Mikrochim Acta. 2022 Sep 8;189(10):378. doi: 10.1007/s00604-022-05476-2.
A new nanozyme (Cu-NADH) is reported composed of Cu-coordinated nicotinamide adenine dinucleotide (NADH) exhibiting laccase-like activity. The Cu-NADH nanozyme had higher heat tolerance and catalytic efficiency than natural laccase, and its catalytic activity can be enhanced by high concentration of Cl ions and it is intensely inhibited by phosphate. Therefore, a colorimetric method based on Cu-NADH and indigo carmine was successfully developed to detect phosphate in water. This method showed an excellent selectivity for phosphate, and it had a linear relationship in the phosphate concentration range 2-50 μM with a detection limit of 0.37 μM. We believe that this example of coordination between metal ions and biomolecules to mimic natural enzymes can inspire more effective and alternative strategies in nanozyme design and expand their use in sensing and determination.
一种新型纳米酶（Cu-NADH）被报道由 Cu 配位的烟酰胺腺嘌呤二核苷酸（NADH）组成，具有漆酶样活性。与天然漆酶相比，Cu-NADH 纳米酶具有更高的耐热性和催化效率，其催化活性可以被高浓度的 Cl 离子增强，并被磷酸盐强烈抑制。因此，成功开发了一种基于 Cu-NADH 和靛蓝胭脂红的比色法来检测水中的磷酸盐。该方法对磷酸盐具有优异的选择性，在磷酸盐浓度范围为 2-50 μM 时具有线性关系，检测限为 0.37 μM。我们相信，这种金属离子与生物分子之间的配位模拟天然酶的例子可以为纳米酶设计提供更有效和替代的策略，并扩展它们在传感和测定中的应用。
