Romasco Tea, Mandrillo Pier Michele, Morsut Erica, Tumedei Margherita, Mandatori Domitilla, Petrini Morena, Curia Maria Cristina, De Angelis Francesco, D'Arcangelo Camillo, Piattelli Adriano, Di Pietro Natalia
Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.
Center for Advanced Studies and Technology-CAST, "G. d'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.
Biomedicines. 2023 Mar 5;11(3):786. doi: 10.3390/biomedicines11030786.
Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h ( < 0.05) and wound closure at 24 h ( < 0.001) of hGF grown on COL, with an increasing trend at all time-points. Furthermore, COL significantly induced the expression of FAK and YAP/TAZ ( < 0.05), thereby promoting the activation of mechanotransduction signaling pathways. Overall, these data suggest that COL, acting as a mechanical bio-scaffold, could represent a useful treatment for gingival rejuvenation and may possibly help in the resolution of oral pathologies.
维持牙周和种植体周围软组织的健康对于牙齿和牙种植体的长期健康至关重要。旨在避免结缔组织改变及相关疾病（如牙周炎和种植体周围炎）的新生物医学策略正在不断发展。其中，基于胶原蛋白的医疗产品已被证明是安全有效的。因此，本研究的目的是评估一种新型可注射医疗器械——Dental SKIN BioRegulation（意大利米兰的古纳股份公司），其由猪源I型胶原蛋白组成，对人牙龈成纤维细胞（hGF）原代培养物的影响。为此，在评估细胞活力（24小时、48小时、72小时和7天）、伤口愈合特性（3小时、6小时、12小时、24小时和48小时）以及机械转导标志物（如FAK、YAP和TAZ）的激活（48小时）之前，将hGF培养在胶原蛋白包被的（COL，100μg/2mL）或未包被的平板（CTRL）上。结果证明，在COL上生长的hGF在48小时时细胞活力显著增加（<0.05），在24小时时伤口闭合显著增加（<0.001），并且在所有时间点都有增加趋势。此外，COL显著诱导FAK和YAP/TAZ的表达（<0.05），从而促进机械转导信号通路的激活。总体而言，这些数据表明，COL作为一种机械生物支架，可能是牙龈年轻化的一种有用治疗方法，并且可能有助于解决口腔疾病。
